{"title":"Advancing plant gene activation with CRISPR-Act3.0.","authors":"Ali Movahedi, Zhiying Mu, Liming Yang","doi":"10.1016/j.tibtech.2025.01.008","DOIUrl":null,"url":null,"abstract":"<p><p>The pioneering plant genome-engineering tool CRISPR-Act3.0 is promising for precise gene activation, but faces challenges, such as unstable structures and inconsistent gene activation in polyploid genomes. Here, we propose strategic modifications to render CRISPR-Act3.0 more resilient and adaptable, especially in complex plant genomes, where conventional methods have struggled.</p>","PeriodicalId":23324,"journal":{"name":"Trends in biotechnology","volume":" ","pages":""},"PeriodicalIF":14.3000,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Trends in biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1016/j.tibtech.2025.01.008","RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The pioneering plant genome-engineering tool CRISPR-Act3.0 is promising for precise gene activation, but faces challenges, such as unstable structures and inconsistent gene activation in polyploid genomes. Here, we propose strategic modifications to render CRISPR-Act3.0 more resilient and adaptable, especially in complex plant genomes, where conventional methods have struggled.
期刊介绍：
Trends in Biotechnology publishes reviews and perspectives on the applied biological sciences, focusing on useful science applied to, derived from, or inspired by living systems.
The major themes that TIBTECH is interested in include:
Bioprocessing (biochemical engineering, applied enzymology, industrial biotechnology, biofuels, metabolic engineering)
Omics (genome editing, single-cell technologies, bioinformatics, synthetic biology)
Materials and devices (bionanotechnology, biomaterials, diagnostics/imaging/detection, soft robotics, biosensors/bioelectronics)
Therapeutics (biofabrication, stem cells, tissue engineering and regenerative medicine, antibodies and other protein drugs, drug delivery)
Agroenvironment (environmental engineering, bioremediation, genetically modified crops, sustainable development).
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