Bioconversion of Liquid and Solid Lipid Waste by Yarrowia lipolytica Yeast: A Study of Extracellular Lipase Biosynthesis and Microbial Lipid Production.

Abstract

This study investigated the capabilities of Yarrowia lipolytica strains to grow in media with different hydrophobic wastes from the meat industry. The yeast growth, cellular lipid accumulation, production of lipases, and degree of utilization of liquid and solid lipid wastes were studied in shaken cultures in media with organic and inorganic nitrogen sources. The effects of the type of waste, initial concentration of carbon source, Yarrowia strain, and inoculum size were investigated in two experimental sets using the Latin Square 5 × 5 design method. Post-frying rapeseed oil from chicken frying was selected as the carbon source to promote biomass growth. In contrast, the solid lipid fraction from meat broths promoted efficient lipid accumulation and yeast lipolytic activity. An initial concentration of the carbon source at 8% m/v stimulated efficient lipid biosynthesis and lipase production, while 2.5% v/v inoculum provided optimal conditions for the growth and utilization of hydrophobic substrates. No significant differences were observed in the particle dispersion of the liquid and solid wastes in the culture media (span = 2.51-3.23). The maximum emulsification index (62%) was observed in the culture of the Y. lipolytica KKP 323 strain in the medium with post-frying rapeseed oil from chicken frying, which was correlated with biosurfactant synthesis. It was concluded that the type of waste, its structure, and its composition affected various physiological yeast responses.