Virucidal potential of oleuropein identified in Castana sativa shell: insight from in vitro and in silico studies.

IF 1.9 4区医学 Q3 ENVIRONMENTAL SCIENCES International Journal of Environmental Health Research Pub Date : 2025-10-01 Epub Date: 2025-03-03 DOI:10.1080/09603123.2025.2472189

Fatma Nouira, Hisham Altayb, Dorra Gharbi, Radhia Bouazizi, Ilaria Frosi, Kamel Chaieb, Adele Papetti, Lamjed Bouslama

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Abstract

In this report, Castanea sativa shell extracts have been investigated for their activity against Herpes Virus type 2 (HSV-2). Among four organic extracts only 75% ethanol extract demonstrated activity against HSV-2 with a 50% inhibitory concentration of 6.45 μg/mL. The study of the mechanism underlying the antiviral activity demonstrated this extract strongly inhibits HSV-2 by direct contact and moderately protects the cell from virus recognition and binding. The active compound has been identified as oleuropein by HPLC-DAD-ESI-MSⁿ. This compound which is widely known in the Oleaceae family has been identified in the chestnut shells for the first time. The in vitro results of the mechanism underlying anti HSV-2 action were confirmed by an in silico study which revealed a strong binding free energy of -9.08 kcal/mol between oleuropein and the glycoprotein D of HSV-2. In summary, our findings suggest that C. sativa shells may constitute a promising natural source of anti-HSV-2 agents.

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蓖麻壳中橄榄苦苷的杀病毒潜力：来自体外和计算机研究的见解。

本文研究了番栗壳提取物对2型疱疹病毒（HSV-2）的抑制作用。在四种有机提取物中，75%乙醇提取物对HSV-2的抑制浓度为6.45 μg/mL，达到50%。抗病毒活性机制的研究表明，该提取物通过直接接触对HSV-2有较强的抑制作用，并适度保护细胞免受病毒识别和结合。经HPLC-DAD-ESI-MSn鉴定为橄榄苦苷。在板栗壳中首次鉴定出该化合物，该化合物是油棕科植物中较为常见的化合物。体外实验结果证实橄榄苦苷与HSV-2糖蛋白D的结合自由能为-9.08 kcal/mol，证实了橄榄苦苷抗HSV-2的作用机制。总之，我们的研究结果表明，苜蓿壳可能是抗hsv -2药物的一个有希望的天然来源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Environmental Health Research 医学-公共卫生、环境卫生与职业卫生

CiteScore

6.70

自引率

3.10%

发文量

134

审稿时长

>12 weeks

期刊介绍： International Journal of Environmental Health Research ( IJEHR ) is devoted to the rapid publication of research in environmental health, acting as a link between the diverse research communities and practitioners in environmental health. Published articles encompass original research papers, technical notes and review articles. IJEHR publishes articles on all aspects of the interaction between the environment and human health. This interaction can broadly be divided into three areas: the natural environment and health – health implications and monitoring of air, water and soil pollutants and pollution and health improvements and air, water and soil quality standards; the built environment and health – occupational health and safety, exposure limits, monitoring and control of pollutants in the workplace, and standards of health; and communicable diseases – disease spread, control and prevention, food hygiene and control, and health aspects of rodents and insects. IJEHR is published in association with the International Federation of Environmental Health and includes news from the Federation of international meetings, courses and environmental health issues.