NtDHS regulates leaf senescence by modulating gene translation in Nicotiana tabacum.

IF 2.6 4区生物学 Q2 PLANT SCIENCES Functional Plant Biology Pub Date : 2025-03-01 DOI:10.1071/FP24294

Junping Gao, Ying Wang, Xinxi He, Long Chen, Shuaibin Wang, Xinyao Zhang, Sirui Zhu, Xiaoxu Li, Xiaonian Yang, Wenxuan Pu, Yuanyuan Li

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引用次数: 0

Abstract

The biochemical and transcriptional regulatory mechanisms of chlorophyll metabolism have been extensively studied, but the translational regulatory mechanisms remain poorly understood. In this study, we found that Nt DHS1 deficiency in N. tabacum resulted in smaller leaves and increased leaf chlorophyll content. Protein content determination experiments revealed that the global protein synthesis of the Ntdhs1 mutant was decreased. A ribosome profiling sequence (Ribo-seq) assay showed that the translation level of genes related to cell growth was significantly reduced, while the translation level of chlorophyll metabolism related genes was significantly increased in Ntdhs1 mutant. Biochemical analysis further demonstrated that Nt DHS interacts with the translation initiation factor Nt eIF5A. Moreover, the Nteif5a1 mutant exhibited phenotypes similar to the Ntdhs1 mutant, including a reduced translation level of cell growth related genes and increased translation level of chlorophyll metabolism related genes. Our studies suggest that the Nt DHS-Nt eIF5A complex regulates leaf senescence by modulating the translation of specific genes.

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来源期刊

Functional Plant Biology 生物-植物科学

CiteScore

5.50

自引率

3.30%

发文量

156

审稿时长

1 months

期刊介绍： Functional Plant Biology (formerly known as Australian Journal of Plant Physiology) publishes papers of a broad interest that advance our knowledge on mechanisms by which plants operate and interact with environment. Of specific interest are mechanisms and signal transduction pathways by which plants adapt to extreme environmental conditions such as high and low temperatures, drought, flooding, salinity, pathogens, and other major abiotic and biotic stress factors. FPB also encourages papers on emerging concepts and new tools in plant biology, and studies on the following functional areas encompassing work from the molecular through whole plant to community scale. FPB does not publish merely phenomenological observations or findings of merely applied significance. Functional Plant Biology is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science. Functional Plant Biology is published in affiliation with the Federation of European Societies of Plant Biology and in Australia, is associated with the Australian Society of Plant Scientists and the New Zealand Society of Plant Biologists.