Fluorescence imaging of RNA and protein in deep tissue

IF 41.7 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Nature biotechnology Pub Date : 2025-03-17 DOI:10.1038/s41587-025-02604-0
Iris Marchal
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深层组织中RNA和蛋白质的荧光成像
通过研究复杂组织中分子成分的排列方式,可以深入了解组织发育和疾病。用荧光显微镜分析三维(3D)组织受到自身荧光和染料的限制。现在,在《科学》杂志上,Gandin等人描述了循环杂交链反应(cycleHCR),使RNA和蛋白质在3D环境下的荧光成像成为可能。CycleHCR介绍了一种优化的条形码系统。作者设计的DNA探针库包含10至25对45-bp的分裂初级探针,每对包含一个独特的14-bp条形码序列,用于将左右读出探针与分裂HCR启动子结合。这种设计仅在特定的左读出和右读出探针都存在时触发HCR。每个颜色通道60个独特探针的组合(30个用于左,30个用于右)产生多达900个不同的条形码,在使用三个通道时编码2,700个目标。CycleHCR不依赖于初始探针移除和成像之间的再杂交,使得该方法比以前的多轮HCR方法更节省时间。
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来源期刊
Nature biotechnology
Nature biotechnology 工程技术-生物工程与应用微生物
CiteScore
63.00
自引率
1.70%
发文量
382
审稿时长
3 months
期刊介绍: Nature Biotechnology is a monthly journal that focuses on the science and business of biotechnology. It covers a wide range of topics including technology/methodology advancements in the biological, biomedical, agricultural, and environmental sciences. The journal also explores the commercial, political, ethical, legal, and societal aspects of this research. The journal serves researchers by providing peer-reviewed research papers in the field of biotechnology. It also serves the business community by delivering news about research developments. This approach ensures that both the scientific and business communities are well-informed and able to stay up-to-date on the latest advancements and opportunities in the field. Some key areas of interest in which the journal actively seeks research papers include molecular engineering of nucleic acids and proteins, molecular therapy, large-scale biology, computational biology, regenerative medicine, imaging technology, analytical biotechnology, applied immunology, food and agricultural biotechnology, and environmental biotechnology. In summary, Nature Biotechnology is a comprehensive journal that covers both the scientific and business aspects of biotechnology. It strives to provide researchers with valuable research papers and news while also delivering important scientific advancements to the business community.
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