Marisa Mercadante, Armin Scheben, Jacob Estrada, Jan Savas-Carstens, William Sullivan, Nicholas Housel, Tatiana Volpari, Jax Hebner, Maria Sapar, Tom Rusielewicz, Frederick J Monsma, Stefan Semrau, Yinan Wang, Laura A Martin
{"title":"A patient-derived ovarian cancer organoid platform to study susceptibility to natural killer cells.","authors":"Marisa Mercadante, Armin Scheben, Jacob Estrada, Jan Savas-Carstens, William Sullivan, Nicholas Housel, Tatiana Volpari, Jax Hebner, Maria Sapar, Tom Rusielewicz, Frederick J Monsma, Stefan Semrau, Yinan Wang, Laura A Martin","doi":"10.1101/2025.03.06.641285","DOIUrl":null,"url":null,"abstract":"<p><p>Intratumoral heterogeneity drives therapy resistance and relapses in advanced stage cancers, such as ovarian cancer. Here, we present a live cell imaging assay using patient-derived ovarian cancer organoids for real time capture and quantification of natural killer cell-mediated apoptotic events in >500 organoids simultaneously. Our assay revealed significant inter- and intratumor response heterogeneity and identified a rare resistant organoid population, opening avenues to test immunomodulatory strategies that overcome resistance.</p>","PeriodicalId":519960,"journal":{"name":"bioRxiv : the preprint server for biology","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11908259/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"bioRxiv : the preprint server for biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1101/2025.03.06.641285","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Intratumoral heterogeneity drives therapy resistance and relapses in advanced stage cancers, such as ovarian cancer. Here, we present a live cell imaging assay using patient-derived ovarian cancer organoids for real time capture and quantification of natural killer cell-mediated apoptotic events in >500 organoids simultaneously. Our assay revealed significant inter- and intratumor response heterogeneity and identified a rare resistant organoid population, opening avenues to test immunomodulatory strategies that overcome resistance.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
