A. Arun Prince Milton , Torik Basar , K. Srinivas , Aleimo G. Momin , Sabia Khan , G. Bhuvana Priya , Samir Das , S. Ghatak , K. Puro
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引用次数: 0
Abstract
In this study, we developed a novel competitive annealing-mediated isothermal amplification (CAMP)-based assay for the detection of the etx gene, which encodes the epsilon toxin of Clostridium perfringens. The assay, performed at 60 °C for 60 min, demonstrated 100 % specificity and was 100 times more sensitive than endpoint PCR.
期刊介绍：
Anaerobe is essential reading for those who wish to remain at the forefront of discoveries relating to life processes of strictly anaerobes. The journal is multi-disciplinary, and provides a unique forum for those investigating anaerobic organisms that cause infections in humans and animals, as well as anaerobes that play roles in microbiomes or environmental processes.
Anaerobe publishes reviews, mini reviews, original research articles, notes and case reports. Relevant topics fall into the broad categories of anaerobes in human and animal diseases, anaerobes in the microbiome, anaerobes in the environment, diagnosis of anaerobes in clinical microbiology laboratories, molecular biology, genetics, pathogenesis, toxins and antibiotic susceptibility of anaerobic bacteria.
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