Highly sensitive chemiluminescence of water-soluble luminol and its application in forensic bloodstain detection†

IF 2.6 3区 化学 Q2 CHEMISTRY, ANALYTICAL Analytical Methods Pub Date : 2025-03-24 DOI:10.1039/D4AY02022G
Mingjiang Wu, Aijiao Liu, Tao Xu, Shuofeng Zhang, Jiayan Lan and Haiyang Wang
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Abstract

Chemiluminescence (CL) provides outstanding analytical performance due to its background-free nature and exceptional sensitivity and selectivity. The forensic luminol chemiluminescence test is one of the most commonly used methods for detecting latent bloodstains. However, its applications are limited by the insolubility of luminol under physiological conditions. In this study, m-carboxy luminol with its hydrophilic design was found to overcome these limitations and showed a chemiluminescence intensity higher than that displayed by any other luminol-based CL assay. The superior CL performance of m-carboxy luminol encouraged us to further apply this derivative for imaging bloodstains, with the results implying excellent application prospects for m-carboxy-luminol-containing imaging solutions in criminal investigations.

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水溶性发光胺的高灵敏度化学发光及其在法医血迹检测中的应用。
化学发光(CL)提供了出色的分析性能,由于其无背景的性质和卓越的灵敏度和选择性。法医发光胺化学发光试验是检测潜在血迹最常用的方法之一。然而,鲁米诺在生理条件下的不溶性限制了其应用。在这项研究中,具有亲水性设计的间羧基鲁米诺克服了这些限制,显示出比任何其他基于鲁米诺的CL测定更高的化学发光强度。间羧基鲁米诺优异的CL性能促使我们进一步将其衍生物应用于血迹成像,结果表明间羧基鲁米诺在刑事侦查中的成像溶液具有良好的应用前景。
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来源期刊
Analytical Methods
Analytical Methods CHEMISTRY, ANALYTICAL-FOOD SCIENCE & TECHNOLOGY
CiteScore
5.10
自引率
3.20%
发文量
569
审稿时长
1.8 months
期刊介绍: Early applied demonstrations of new analytical methods with clear societal impact
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