Intrinsically disordered arginine-glycine repeat domains tune sub-nucleolar compartmentalization in Caenorhabditis elegans.

IF 5.1 3区生物学 Q2 GENETICS & HEREDITY Genetics Pub Date : 2025-06-04 DOI:10.1093/genetics/iyaf067

Emily L Spaulding, Dustin L Updike

引用次数: 0

Abstract

Intrinsically disordered arginine-glycine (RG) repeat domains are enriched in multilayered biomolecular condensates such as the nucleolus. Caenorhabditis elegans nucleolar RG repeats are dispensable for nucleolar accumulation and instead contribute to the organization of sub-nucleolar compartments. The sufficiency of RG repeats to facilitate sub-nucleolar compartmentalization is unclear. In this study, we drive expression of full-length RG repeats in the C. elegans germline to test their ability to localize to nucleoli and organize into nucleolar sub-compartments in vivo. We find that repeats accumulate within germ cell nucleoli but do not enrich in the correct sub-compartment. Our results suggest that RG repeats may indirectly influence nucleolar organization by creating an environment favorable for sub-nucleolar compartmentalization of proteins primarily based on their function within the nucleolus.

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本质上无序的精氨酸-甘氨酸（RG）重复结构域可调整优雅小鼠的核下区隔。

内在无序精氨酸-甘氨酸（RG）重复结构域富集于核仁等多层生物分子凝聚体中。秀丽隐杆线虫核仁RG重复序列在核仁积累中是不可缺少的，而是有助于亚核仁区室的组织。RG重复序列促进亚核仁区隔的充分性尚不清楚。在这项研究中，我们驱动全长RG重复序列在秀丽隐杆线虫种系中的表达，以测试它们在体内定位到核仁并组织成核仁亚室的能力。我们发现重复序列在生殖细胞核仁内积累，但在正确的亚室中不富集。我们的研究结果表明，RG重复序列可能间接影响核仁组织，通过创造一个有利于蛋白质亚核仁区隔化的环境，主要基于它们在核仁内的功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genetics GENETICS & HEREDITY-

CiteScore

6.90

自引率

6.10%

发文量

177

审稿时长

1.5 months

期刊介绍： GENETICS is published by the Genetics Society of America, a scholarly society that seeks to deepen our understanding of the living world by advancing our understanding of genetics. Since 1916, GENETICS has published high-quality, original research presenting novel findings bearing on genetics and genomics. The journal publishes empirical studies of organisms ranging from microbes to humans, as well as theoretical work. While it has an illustrious history, GENETICS has changed along with the communities it serves: it is not your mentor''s journal. The editors make decisions quickly – in around 30 days – without sacrificing the excellence and scholarship for which the journal has long been known. GENETICS is a peer reviewed, peer-edited journal, with an international reach and increasing visibility and impact. All editorial decisions are made through collaboration of at least two editors who are practicing scientists. GENETICS is constantly innovating: expanded types of content include Reviews, Commentary (current issues of interest to geneticists), Perspectives (historical), Primers (to introduce primary literature into the classroom), Toolbox Reviews, plus YeastBook, FlyBook, and WormBook (coming spring 2016). For particularly time-sensitive results, we publish Communications. As part of our mission to serve our communities, we''ve published thematic collections, including Genomic Selection, Multiparental Populations, Mouse Collaborative Cross, and the Genetics of Sex.