{"title":"Saikosaponin D, a triterpene saponin from <i>Bupleurum falcatum</i>, restores circadian rhythm and attenuates hepatic fibrosis via Esr1-Per2 axis.","authors":"Yu-Gang Fu, Jun-Min Wang, Si-Min Gu, Chong Chen, Yan-Ping Wang, Yi-Yuan Zheng, Yong Li","doi":"10.1080/10286020.2025.2482068","DOIUrl":null,"url":null,"abstract":"<p><p>Hepatic fibrosis, a precursor to chronic liver diseases, is linked to circadian rhythm disruptions. This study investigates the anti-fibrotic effects of saikosaponin D (SSd) from <i>Bupleurum falcatum L</i>. and its regulation of circadian genes. Transcriptomic and single-cell analyses revealed circadian gene dysregulation, particularly Period 2 (Per2), in liver fibrosis and hepatic stellate cells. SSd upregulated Per2 transcription via estrogen receptor 1 (Esr1), as confirmed by siRNA, real-time quantitative polymerase chain reaction, and Western blot analyses. <i>In vivo</i>, SSd alleviated fibrosis and hepatitis, increasing Esr1 but not Per2 protein levels. These findings suggest SSd exerts anti-fibrotic effects by modulating Esr1-mediated Per2 transcription.</p>","PeriodicalId":15180,"journal":{"name":"Journal of Asian Natural Products Research","volume":" ","pages":"1-18"},"PeriodicalIF":1.3000,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Asian Natural Products Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/10286020.2025.2482068","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
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Abstract
Hepatic fibrosis, a precursor to chronic liver diseases, is linked to circadian rhythm disruptions. This study investigates the anti-fibrotic effects of saikosaponin D (SSd) from Bupleurum falcatum L. and its regulation of circadian genes. Transcriptomic and single-cell analyses revealed circadian gene dysregulation, particularly Period 2 (Per2), in liver fibrosis and hepatic stellate cells. SSd upregulated Per2 transcription via estrogen receptor 1 (Esr1), as confirmed by siRNA, real-time quantitative polymerase chain reaction, and Western blot analyses. In vivo, SSd alleviated fibrosis and hepatitis, increasing Esr1 but not Per2 protein levels. These findings suggest SSd exerts anti-fibrotic effects by modulating Esr1-mediated Per2 transcription.
期刊介绍：
The Journal of Asian Natural Products Research (JANPR) publishes chemical and pharmaceutical studies in the English language in the field of natural product research on Asian ethnic medicine. The journal publishes work from scientists in Asian countries, e.g. China, Japan, Korea and India, including contributions from other countries concerning natural products of Asia. The journal is chemistry-orientated. Major fields covered are: isolation and structural elucidation of natural constituents (including those for non-medical uses), synthesis and transformation (including biosynthesis and biotransformation) of natural products, pharmacognosy, and allied topics. Biological evaluation of crude extracts are acceptable only as supporting data for pure isolates with well-characterized structures.
All published research articles in this journal have undergone rigorous peer review, based on initial editor screening and anonymized refereeing by at least two expert referees.
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