Intramembrane fluidity increases when the cyclohexyl ring of retinal binds to rod outer-segment membranes.

D L Vessell
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Abstract

The spin-label 5-4',4'-dimethyloxazolidinyl-N-oxy stearate (5-doxyl-stearate) exists in a viscous environment when it is incorporated into bleached rod outer segment membranes (ROSM). Addition of the cyclohexyl ring moiety of retinal to ROSM produced a large increase in intramembrane fluidity. A decrease in both the order parameter S (from 0.5 to 0.14) and the rotational correlation time (from 4.0 to 2.0 nsec) gives an estimate of the magnitude of change. Other ring analogs produced similar fluidity changes. The extent of their action depended on their structural similarity to the cyclohexyl of retinal. The pH range in which the cyclohexyl ring produced the increase in ROSM fluidity was 6.8-9.5.

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当视网膜环己基环与杆外节膜结合时,膜内流动性增加。
自旋标记5- 4,4 '-二甲基氧杂唑烷基- n-氧硬脂酸酯(5-羟硬脂酸酯)在掺入漂白棒外段膜(ROSM)时存在于粘性环境中。将视网膜的环己基环部分添加到ROSM中,膜内流动性大大增加。序参数S(从0.5到0.14)和旋转相关时间(从4.0到2.0 nsec)的减少给出了变化幅度的估计。其他环类似物也产生了类似的流动性变化。它们的作用程度取决于它们与视网膜环己基的结构相似性。环己基环增加ROSM流动性的pH值范围为6.8 ~ 9.5。
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