Some properties of proline-sRNA synthetase from rat liver

Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation Pub Date : 1966-10-17 DOI:10.1016/0926-6593(66)90153-6

Clark Bublitz

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引用次数: 9

Abstract

1.
1. Some of the properties of a proline-sRNA synthetase from rat liver have been studied by either the hydroxamate assay or the proline-dependent ³²PP_i-ATP exchange.
2.
2. The enzyme was noticeably stabilized by sucrose. Although all preparations were stimulated by mercaptoethanol, some aged preparations had an absolute requirement for added mercaptan for activity.
3.
3. The enzyme required either magnesium, manganese, or calcium for activity. Hydroxamate formation was inhibited by PP_i.
4.
4. [¹⁴C]Proline hydroxamate formation was inhibited by 3,4-dehydroproline; azetidine carboxylic acid; thiazolidine carboxylic acid; 2-methyl-, 2-ethyl-, or 2-propylthiazolidine carboxylic acid; thiazolidine; and mercaptoethylamine. Thiazolidine and mercaptoethylamine were competitive inhibitors of proline.
5.
5. The specific activity of extracts from younger rats was higher than that from older rats. Starvation also increased the activity of the extracts.

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大鼠肝脏脯氨酸- srna合成酶的一些性质

1.1. 从大鼠肝脏提取的脯氨酸- srna合成酶的一些特性已经通过羟酸测定或脯氨酸依赖的32PPi-ATP交换进行了研究。蔗糖明显地稳定了这种酶。虽然所有的制剂都受到巯基乙醇的刺激，但有些陈化制剂的活性绝对需要添加巯基乙醇。这种酶需要镁、锰或钙才能发挥活性。PPi.4.4抑制羟酸酯的形成。[14C] 3,4-脱氢脯氨酸抑制脯氨酸羟酸酯的形成;氮杂丁羧酸;噻唑烷羧酸;2-甲基、2-乙基或2-丙基噻唑烷羧酸;thiazolidine;和mercaptoethylamine。噻唑烷和巯基乙胺是脯氨酸的竞争性抑制剂。年轻大鼠提取物的比活性高于年老大鼠提取物。饥饿也增加了提取物的活性。

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Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation

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