Adenosine triphosphatase activity of heart mitochondria of the turtle, Chrysemys picta

Comparative biochemistry and physiology Pub Date : 1970-11-01 DOI:10.1016/0010-406X(70)90953-9

A.J. Rotermund , C.A. Privitera

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引用次数: 4

Abstract

1.
1. ATPase activity has been estimated in heart mitochondria of the fresh-water turtle, Chrysemys picta.
2.
2. This activity is Mg²⁺-dependent and stimulated by the presence of DNP.
3.
3. The pH optima of ATPase activities are 8·5 with Mg²⁺ ions, and 7·4 in the presence of DNP.
4.
4. Differential responses to inhibitors are evident in the presence of added Mg²⁺ ions or DNP, with DNP-stimulated activities being sensitive to a wider spectrum of inhibitors and to the aging process.
5.
5. Bovine serum albumin, but not EDTA, effectively preserves DNP-stimulated ATPase activity during aging. A causal relationship with fatty acids is implied.
6.
6. These data may be used to support either a one or a two enzyme hypothesis, with the latter being favored.

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鳖心脏线粒体腺苷三磷酸酶活性的研究

1.1. 淡水龟心脏线粒体中atp酶活性已被估计，Chrysemys picture .2.2。这种活性依赖于Mg2+，并受到DNP.3.3的刺激。Mg2+离子作用下atp酶活性的pH最优值为8.5,DNP.4.4存在下atp酶活性的pH最优值为7.4。在添加Mg2+离子或DNP的情况下，对抑制剂的不同反应是明显的，DNP刺激的活性对更广泛的抑制剂和老化过程敏感。牛血清白蛋白，而不是EDTA，在衰老过程中有效地保持dnp刺激的atp酶活性。隐含着与脂肪酸的因果关系。这些数据可用于支持单酶假说或双酶假说，后者更受青睐。

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Comparative biochemistry and physiology

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