{"title":"The dissolution and fractionation of bovine sclera","authors":"Jerome Rotstein M.D. , Jules Seltzer M.S.","doi":"10.1016/0926-6534(65)90005-9","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. A method for dissolving and fractionating bovine sclera has been described. High speed homogenization, utilizing double water extraction, separates out the protein-polysaccharide from the sclera. The residue is disolved with pepsin (EC 3.4.4.1) and trypsin (EC 3.4.4.4) and fractionated by dialysis and cold alcohol precipitation.</p></span></li><li><span>2.</span><span><p>2. Chondroitin sulfates A, B and C are found as protein-polysaccharide complexes in bovine sclera. No keratosulfate was found in the tissue.</p></span></li><li><span>3.</span><span><p>3. No <em>N</em>-acetyl neuraminic acid is present in bovine sclera.</p></span></li><li><span>4.</span><span><p>4. Comparison of the fractions of the bovine sclera and bovine nasal cartilage reveals that the tissues are different in composition and structure.</p></span></li></ul></div>","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1965-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90005-9","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926653465900059","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4
Abstract
1.
1. A method for dissolving and fractionating bovine sclera has been described. High speed homogenization, utilizing double water extraction, separates out the protein-polysaccharide from the sclera. The residue is disolved with pepsin (EC 3.4.4.1) and trypsin (EC 3.4.4.4) and fractionated by dialysis and cold alcohol precipitation.
2.
2. Chondroitin sulfates A, B and C are found as protein-polysaccharide complexes in bovine sclera. No keratosulfate was found in the tissue.
3.
3. No N-acetyl neuraminic acid is present in bovine sclera.
4.
4. Comparison of the fractions of the bovine sclera and bovine nasal cartilage reveals that the tissues are different in composition and structure.
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