[Effector influence of oxygen-containing C1 compounds in the cultivation of the methanotrophic bacterial strain GB 25].

J D Schneider, K D Wendlandt, E Brühl, G Mirschel
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Abstract

Addition of oxygen-containing C1-compounds to chemostat cultures of GB 25 increases both the yield of biomass and the specific growth rate. At optimum concentrations the catalytic activity of these compounds increases with increasing growth rates. Their influence on maintenance coefficients and maximum yield coefficients decreases in the order CH3OH greater than CO2 greater than HCOOH greater than HCHO. This result together with spectrophotometric NADH determinations suggests that the NADH pool determines the balance between the assimilatory and oxidative utilization of formaldehyde.

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[含氧C1化合物对甲烷营养菌株gb25培养的效应影响]。
在恒化培养物中添加含氧c1化合物,既提高了生物量产量,又提高了比生长率。在最佳浓度下,这些化合物的催化活性随着生长速率的增加而增加。它们对维持系数和最大产率系数的影响依次为CH3OH > CO2 > HCOOH > HCHO。这一结果与分光光度法测定NADH表明,NADH池决定了甲醛吸收和氧化利用之间的平衡。
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