Salmonella and mammalian-cell mutagenicity of 3-chloro-4-(chloromethyl)-5-hydroxy-2(5H)-furanone

Mutation Research Letters Pub Date : 1995-09-01 DOI:10.1016/0165-7992(95)90021-7

Minna Niittykoski, Juha M.T. Hyttinen, Kristian Jansson

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引用次数: 11

Abstract

3-Chloro-4-(chloromethyl)5-hydroxy-2(5H)-furanone (CMCF), a chlorine disinfection by-product in drinking water, was mutagenic in the Salmonella/his (Ames) assay for both base-pair substitution strains (TA1535, TA100 TA102) and frameshift strains (TA97, TA98) with the highest mutagenic response observed in strain TA100 (1292 revertants/μg). The presence in TA100 of pKM101 plasmid, which enhances error-phone DNA repair, greatly increased susceptibility of CMCF mutagenicity relative to the isogenic strain TA1535 lacking pKM101. In the Chinese hamster ovary (CHO) cell hprt (6-thioguanine resistance) locus assay, the mutagenicity of CMCF (1.04 mutants/10⁶ clonable cells per μg/ml) was barely detectable because of the low mutagenicity/cytotoxicity ratio. From the present experiments it appears that CMCF acts in a manner similar to that of another drinking water mutagen, 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX). However, CMFC appears to be a less potent mutagen in vitro than MX

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3-氯-4-(氯甲基)-5-羟基-2(5H)-呋喃酮对沙门氏菌和哺乳动物细胞的致突变性

饮用水氯消毒副产物3-氯-4-(氯甲基)5-羟基-2(5H)-呋喃酮(CMCF)对碱基对置换菌株(TA1535、TA100、TA102)和移码菌株(TA97、TA98)均具有致突变性，其中菌株TA100的致突变性最高(1292个/μg)。与缺乏pKM101的等基因菌株TA1535相比，TA100中存在pKM101质粒，增强了错误电话DNA修复，大大增加了CMCF致突变性的易感性。在中国仓鼠卵巢(CHO)细胞hprt(6-硫鸟嘌呤抗性)位点测定中，由于CMCF的致突变性/细胞毒性比较低，几乎检测不到其致突变性(1.04个突变体/106个克隆细胞/ μg/ml)。从目前的实验来看，CMCF的作用方式类似于另一种饮用水诱变剂3-氯-4-(二氯甲基)-5-羟基-2(5H)-呋喃酮(MX)。然而，CMFC在体外的诱变效果似乎不如MX

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Mutation Research Letters

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