de Souza Sandro José, Madaio Michael P., Neto Luis Juliano, Brentani Ricardo Renzo
{"title":"A Monoclonal Autoantibody against a Complementary Peptide Recognizes Interstitial Collagenase","authors":"de Souza Sandro José, Madaio Michael P., Neto Luis Juliano, Brentani Ricardo Renzo","doi":"10.1006/immu.1994.1052","DOIUrl":null,"url":null,"abstract":"<div><p>A monoclonal autoantibody (mab 16) against the complementary peptide TKKTLRT, which was deduced from the collagenase-sensitive site in collagen, is described. Mab 16 recognized interstitial collagenase as visualized by ELISA and immunoprecipitation assays. Moreover, mab 16 was able to inhibit partially the collagenolytic activity of keratinocyte supernatant. Finally, it was possible to immunopurify collagenase using a mab 16/Sepharose column.</p></div>","PeriodicalId":79341,"journal":{"name":"ImmunoMethods","volume":"5 2","pages":"Pages 172-176"},"PeriodicalIF":0.0000,"publicationDate":"1994-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/immu.1994.1052","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ImmunoMethods","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1058668784710527","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4
Abstract
A monoclonal autoantibody (mab 16) against the complementary peptide TKKTLRT, which was deduced from the collagenase-sensitive site in collagen, is described. Mab 16 recognized interstitial collagenase as visualized by ELISA and immunoprecipitation assays. Moreover, mab 16 was able to inhibit partially the collagenolytic activity of keratinocyte supernatant. Finally, it was possible to immunopurify collagenase using a mab 16/Sepharose column.
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