Factors of Ehrlich Ascites Tumor resistance to oxidative stress.

Abstract

Ehrlich Ascites Tumor (EAT) cells, compared to liver, were less susceptible to peroxidation (measured by the TBARS method) induced by Fe2+/ascorbate, ADP/Fe2+ and H2O2/Fe2+/ascorbate. Higher thiobarbituric acid reagent products TBARS levels were found with Fe2+/ascorbate or after ultrasonication induced peroxidation in lipids obtained from Ehrlich cells compared to EAT cell homogenate with the same lipid content. This suggests that non-lipid and structurally membrane antioxidants factors are of less importance for induction by Fe2+/ascorbate than by ultrasonication. The resistance to induced lipid peroxidation (LPO) in EAT cells is associated with the lower level of lipids, the lower double-bond index, the lower level of fatty acids reacting with TBA, as well as with the relatively higher level of alpha-tocopherol per mg lipid in the EAT cells as compared to the liver.