R Bristow, J Byrne, J Squirell, H Trencher, T Carter, B Rodgers, E Saman, J Duncan
{"title":"Human cyclophilin has a significantly higher affinity for HIV-1 recombinant p55 than p24.","authors":"R Bristow, J Byrne, J Squirell, H Trencher, T Carter, B Rodgers, E Saman, J Duncan","doi":"10.1097/00042560-199904010-00002","DOIUrl":null,"url":null,"abstract":"<p><p>The ability of cyclophilin to bind a panel of recombinant HIV-gag proteins was assessed using sensitive, quantitative, sandwich enzyme-linked immunosorbant assays (ELISAs). Significantly higher binding to cyclophilin was observed when recombinants contained at least 12 carboxy-terminal amino acids of p17 in addition to p24 sequences. These results indicate that the carboxy-terminus of p17 is important for optimal binding of cyclophilin to p24 and support the theory that cyclophilin acts on the uncleaved HIV-1 gag (p17-p24) precursor.</p>","PeriodicalId":14731,"journal":{"name":"Journal of acquired immune deficiency syndromes and human retrovirology : official publication of the International Retrovirology Association","volume":"20 4","pages":"334-6"},"PeriodicalIF":0.0000,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"17","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of acquired immune deficiency syndromes and human retrovirology : official publication of the International Retrovirology Association","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1097/00042560-199904010-00002","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 17
Abstract
The ability of cyclophilin to bind a panel of recombinant HIV-gag proteins was assessed using sensitive, quantitative, sandwich enzyme-linked immunosorbant assays (ELISAs). Significantly higher binding to cyclophilin was observed when recombinants contained at least 12 carboxy-terminal amino acids of p17 in addition to p24 sequences. These results indicate that the carboxy-terminus of p17 is important for optimal binding of cyclophilin to p24 and support the theory that cyclophilin acts on the uncleaved HIV-1 gag (p17-p24) precursor.
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