[Comparative study on biological characteristics and ultrastructure of the fibroblasts derived from normal skin, hypertrophic scar and keloidin in vitro culture].

Abstract

Objective: This study was to investigate the in vitro cultivation, biological characteristics and ultrastructure of the fibroblasts derived from normal skin, hypertrophic scar and keloid, to elucidate the value of their application.

Method: A comparative study was performed on cell proliferation, cell morphology, cytogenetic feature and cell ultrastructure of fibroblasts from normal skin, hypertrophic scar and keloid by means of the technique of in vitro culture.

Result: The results indicated that the fibroblasts isolated from normal skin, hypertrophic scar and keloid exhibited similar morphology and growth rates. The cytogenetic feature and cell ultrastructure were also alike.

Conclusion: According to our study and that of others, we conclude that it is entirely feasible to establish an in vitro culture model of fibroblasts from normal skin for investigation, prevention and treatment of scars.