[Function of auxin-binding protein gene during cucumber fruit development].

Shi yan sheng wu xue bao Pub Date : 2004-12-01
Ji Gang Bai, Xiu Juan Wang, Qian Xun Yin, Yu Zhen Xu, Rui Xue Zhao
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Abstract

A cDNA fragment that encodes auxin-binding protein 1 was amplified by, reverse polymerase chain reaction from ovary of cucumber. Its expression signals were weak in the ovary of 1 d before anthesis, while got strong in 2, 4 and 6 d after pollination. Among the unpollinated ovary of 2 d after anthesis, those that got enlarged had strong expression signals; the others that were wilting had weak signals. This indicated that auxin-binding protein 1 gene possibly play a role in cucumber fruit development. When auxin-binding protein 1 gene of Arabidopsis was transformed into cucumber, the parthenocarpic rate of transgenic plants was 31.7%, higher than the control. This result showed that the sensibility to auxin was increased in transgenic plants.

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[生长素结合蛋白基因在黄瓜果实发育中的作用]。
利用逆转录酶链反应从黄瓜子房中扩增出生长素结合蛋白1的cDNA片段。其表达信号在花前1 d在子房较弱,在授粉后2、4和6 d较强。在开花后2 d的未授粉子房中,增大子房的表达信号较强;其他枯萎的细胞信号很弱。这表明生长素结合蛋白1基因可能在黄瓜果实发育中起作用。将拟南芥生长素结合蛋白1基因转化到黄瓜中,转基因植株的单性繁殖率为31.7%,高于对照。结果表明,转基因植株对生长素的敏感性增强。
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