Genetic diversity of Histoplasma capsulatum strains in Brazil

FEMS immunology and medical microbiology Pub Date : 2005-09-01 DOI:10.1016/j.femsim.2005.05.018

Rosely M. Zancopé-Oliveira, Patrícia Morais e Silva Tavares, Mauro de Medeiros Muniz

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引用次数: 26

Abstract

This study establishes the genetic relatedness among Brazilian Histoplasma capsulatum samples obtained from different sources. A PCR-based random amplified polymorphic DNA (RAPD) assay was used to delineate polymorphisms among isolates in geographically diverse regions in Brazil. RAPD fingerprints revealed distinct DNA profiles and provided a high level of discrimination among H. capsulatum strains from different locations. Cluster I was composed of H. capsulatum isolates from the northeast region. The majority of strains from southeast and south were categorized as major cluster II. The strain 84564 from Rio de Janeiro State showed no genetic correlation to any of the isolates from the same state. The RAPD patterns of H. capsulatum isolates from Goias (Cluster III) were unrelated to DNA fingerprints observed among the other H. capsulatum strains (48% similarity). This study is the first report that stratifies the clusters of H. capsulatum strains from Brazil by molecular typing and associates them with the geographical origin.

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巴西荚膜组织浆体菌株的遗传多样性

本研究建立了从不同来源获得的巴西荚膜组织浆体样本之间的遗传相关性。一种基于pcr的随机扩增多态性DNA (RAPD)测定方法被用于描述巴西不同地理区域分离株的多态性。RAPD指纹图谱显示了不同产地荚膜荚膜荚膜菌的不同DNA图谱，具有较高的区分能力。聚类1由东北地区荚膜荚膜荚膜菌分离株组成。大部分来自东南部和南部的毒株被归为主要聚类II。来自巴西里约热内卢州的菌株84564与来自同一州的任何分离株没有遗传相关性。h·戈亚斯capsulatum分离的RAPD模式(集群III)与DNA指纹观察其他h . capsulatum株(48%相似)。本研究首次用分子分型方法对巴西荚膜囊胞菌群进行分型，并将其与地理起源联系起来。

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来源期刊

FEMS immunology and medical microbiology 医学-传染病学

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3-8 weeks