Identification of the infectious source of an unusual outbreak of histoplasmosis, in a hotel in Acapulco, state of Guerrero, Mexico

Maria Lucia Taylor , Guillermo M. Ruíz-Palacios , María del Rocío Reyes-Montes , Gabriela Rodríguez-Arellanes , Laura E. Carreto-Binaghi , Esperanza Duarte-Escalante , Aurora Hernández-Ramírez , Armando Pérez , Roberto O. Suárez-Alvarez , Yuri A. Roldán-Aragón , Rafael Romero-Martínez , Jorge H. Sahaza-Cardona , José Sifuentes-Osornio , Luis E. Soto-Ramírez , Gabriela R. Peña-Sandoval
{"title":"Identification of the infectious source of an unusual outbreak of histoplasmosis, in a hotel in Acapulco, state of Guerrero, Mexico","authors":"Maria Lucia Taylor ,&nbsp;Guillermo M. Ruíz-Palacios ,&nbsp;María del Rocío Reyes-Montes ,&nbsp;Gabriela Rodríguez-Arellanes ,&nbsp;Laura E. Carreto-Binaghi ,&nbsp;Esperanza Duarte-Escalante ,&nbsp;Aurora Hernández-Ramírez ,&nbsp;Armando Pérez ,&nbsp;Roberto O. Suárez-Alvarez ,&nbsp;Yuri A. Roldán-Aragón ,&nbsp;Rafael Romero-Martínez ,&nbsp;Jorge H. Sahaza-Cardona ,&nbsp;José Sifuentes-Osornio ,&nbsp;Luis E. Soto-Ramírez ,&nbsp;Gabriela R. Peña-Sandoval","doi":"10.1016/j.femsim.2005.05.017","DOIUrl":null,"url":null,"abstract":"<div><p>Three isolates of <span><em>Histoplasma capsulatum</em></span> were identified from mice lung, liver, and spleen inoculated with soil samples of the X hotel’s ornamental potted plants that had been fertilized with organic material known as compost. The presence of <em>H. capsulatum</em><span><span> in the original compost was detected using the dot-enzyme-linked immunosorbent assay. Nested-PCR, using a specific protein Hcp100 coding gene sequence, confirmed the fungal identification associated with an unusual </span>histoplasmosis outbreak in Acapulco. Although, diversity between the </span><em>H. capsulatum</em> isolate from the hotel and some clinical isolates from Guerrero (positive controls) was observed using random amplification of polymorphic DNA based-PCR, sequence analyses of <em>H-anti</em> and <em>ole</em> fragment genes revealed a high homology (92–99%) between them.</p></div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"45 3","pages":"Pages 435-441"},"PeriodicalIF":0.0000,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.05.017","citationCount":"40","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"FEMS immunology and medical microbiology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0928824405001537","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 40

Abstract

Three isolates of Histoplasma capsulatum were identified from mice lung, liver, and spleen inoculated with soil samples of the X hotel’s ornamental potted plants that had been fertilized with organic material known as compost. The presence of H. capsulatum in the original compost was detected using the dot-enzyme-linked immunosorbent assay. Nested-PCR, using a specific protein Hcp100 coding gene sequence, confirmed the fungal identification associated with an unusual histoplasmosis outbreak in Acapulco. Although, diversity between the H. capsulatum isolate from the hotel and some clinical isolates from Guerrero (positive controls) was observed using random amplification of polymorphic DNA based-PCR, sequence analyses of H-anti and ole fragment genes revealed a high homology (92–99%) between them.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
在墨西哥格雷罗州阿卡普尔科的一家酒店内,组织胞浆菌病异常暴发的传染源鉴定
用X酒店的观赏盆栽植物的土壤样本接种后,用有机材料堆肥施肥,从小鼠的肺部、肝脏和脾脏中分离出三株荚膜组织浆体。采用点酶联免疫吸附法检测原堆肥中荚膜荚膜菌的存在。利用特定蛋白Hcp100编码基因序列的巢式pcr证实了与阿卡普尔科不寻常的组织胞浆菌病暴发相关的真菌鉴定。虽然利用随机扩增的多态性DNA - pcr方法观察了从酒店分离的荚膜荚膜菌与格雷罗(阳性对照)临床分离的荚膜荚膜菌之间的差异,但对H-anti和ole片段基因的序列分析显示它们之间具有很高的同源性(92% - 99%)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
审稿时长
3-8 weeks
期刊最新文献
Structural, serological, and genetic characterization of the O-antigen of Providencia alcalifaciens O40. Development and evaluation of loop-mediated isothermal amplification (LAMP) for the rapid diagnosis of Penicillium marneffei in archived tissue samples. Molecular characteristics of community-acquired, methicillin-resistant Staphylococcus aureus isolated from Chinese children. Autoactivation of the AggR regulator of enteroaggregative Escherichia coli in vitro and in vivo. Vaccination prevents Helicobacter pylori-induced alterations of the gastric flora in mice.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1