Lenka Sedlaříková, Lenka Bešše, Soňa Novosadová, Veronika Kubaczková, Lenka Radová, Michal Staník, Marta Krejčí, Roman Hájek, Sabina Ševčíková
{"title":"MicroRNAs in urine are not biomarkers of multiple myeloma.","authors":"Lenka Sedlaříková, Lenka Bešše, Soňa Novosadová, Veronika Kubaczková, Lenka Radová, Michal Staník, Marta Krejčí, Roman Hájek, Sabina Ševčíková","doi":"10.1186/s12952-015-0035-7","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>In this study, we aimed to identify microRNA from urine of multiple myeloma patients that could serve as a biomarker for the disease.</p><p><strong>Results: </strong>Analysis of urine samples was performed using Serum/Plasma Focus PCR MicroRNA Panel (Exiqon) and verified using individual TaqMan miRNA assays for qPCR. We found 20 deregulated microRNA (p < 0.05); for further validation, we chose 8 of them. Nevertheless, only differences in expression levels of miR-22-3p remained close to statistical significance.</p><p><strong>Conclusions: </strong>Our preliminary results did not confirm urine microRNA as a potential biomarker for multiple myeloma.</p>","PeriodicalId":73849,"journal":{"name":"Journal of negative results in biomedicine","volume":"14 ","pages":"16"},"PeriodicalIF":0.0000,"publicationDate":"2015-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12952-015-0035-7","citationCount":"7","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of negative results in biomedicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s12952-015-0035-7","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 7
Abstract
Background: In this study, we aimed to identify microRNA from urine of multiple myeloma patients that could serve as a biomarker for the disease.
Results: Analysis of urine samples was performed using Serum/Plasma Focus PCR MicroRNA Panel (Exiqon) and verified using individual TaqMan miRNA assays for qPCR. We found 20 deregulated microRNA (p < 0.05); for further validation, we chose 8 of them. Nevertheless, only differences in expression levels of miR-22-3p remained close to statistical significance.
Conclusions: Our preliminary results did not confirm urine microRNA as a potential biomarker for multiple myeloma.
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