Assessing Healthspan and Lifespan Measures in Aging Mice: Optimization of Testing Protocols, Replicability, and Rater Reliability

Q1 Agricultural and Biological Sciences Current protocols in mouse biology Pub Date : 2018-06-20 DOI:10.1002/cpmo.45

Stacey J. Sukoff Rizzo, Laura C. Anderson, Torrian L. Green, Tracy McGarr, Gaylynn Wells, Shawn S. Winter

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引用次数: 45

Abstract

The relationship between chronological age (lifespan) and biological age (healthspan) varies amongst individuals. Understanding the normal trajectory and characteristic traits of aging mice throughout their lifespan is important for selecting the most reliable and reproducible measures to test hypotheses. The protocols herein describe assays used for aging studies at The Jackson Laboratory's Mouse Neurobehavioral Phenotyping Facility and include assessments of frailty, cognition, and sensory (hearing, vision, olfaction), motor, and fine motor function that can be used for assessing phenotypes in aged mice across their lifespan as well as provide guidance for setting up and validating these behavioral measures. Researchers aiming to study aging phenotypes require access to aged mice as a reference when initiating these types of studies in order to observe normal aging characteristics that cannot be observed in young adult mouse populations. © 2018 by John Wiley & Sons, Inc.

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评估衰老小鼠的健康寿命和寿命:测试方案的优化、可重复性和更高的可靠性

实际年龄(寿命)和生理年龄(健康寿命)之间的关系因人而异。了解衰老小鼠在其整个生命周期中的正常轨迹和特征特征对于选择最可靠和可重复的测量方法来检验假设非常重要。本文的方案描述了在杰克逊实验室的小鼠神经行为表型设施中用于衰老研究的分析，包括虚弱，认知，感觉(听觉，视觉，嗅觉)，运动和精细运动功能的评估，可用于评估老年小鼠在其整个生命周期中的表型，并为建立和验证这些行为测量提供指导。旨在研究衰老表型的研究人员在启动这些类型的研究时需要获得老年小鼠作为参考，以便观察在年轻成年小鼠群体中无法观察到的正常衰老特征。©2018 by John Wiley &儿子,Inc。

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Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.