{"title":"[Chemical Constituents of the Endophytic Fungus Rhinocladiella sp. lgt-3 from Tripterygium wilfordii].","authors":"Cheng Peng, Zhong-duo Yang","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To study the chemical constituents and monoamine oxidase inhibitory activity of Rhinocladiella sp. lgt-3,which was an endophytic fungus isolated from Tripterygium wilfordii.</p><p><strong>Methods: </strong>Compounds were isolated from by various column chromatography. The structures were elucidated by various spectral data. The activity was tested by using 96-well micro-plated method.</p><p><strong>Results: </strong>10 compounds were isolated and identified as 3,4-dihydro-3,4,8-trihydroxy-1( 2H)-naphthalenone( 1),( 3R,4R)-4-hydroxymellein( 2),O-methylmellein( 3),4-( 2-hydroxybutynoxy) benzoic acid( 4),2,4-dihydroxy-6-(( R)-4-hydroxy-2-oxopentyl)-3-methylbenzaldehyde( 5),( Z)-N-( 4-hydroxystyryl) formamide( 6), aspterric acid( 7),bufotenin( 8),5-hydroxy-N,N-dimethyltryptamine-N-oxide( 9)and 5-methoxy-N,N-dimethyltryptamine( 10). Compounds 1,8 ~ 10 showed moderate anti-monoamine oxidase activity with IC50 values of 32,36,50,56 μmol/L, respectively.</p><p><strong>Conclusion: </strong>Compounds 3 ~ 10 are isolated from Rhinocladiella genus for the first time and the anti-monoamine oxidase activity of compound 1 are reported for the first time.</p>","PeriodicalId":15312,"journal":{"name":"中药材","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"中药材","FirstCategoryId":"3","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To study the chemical constituents and monoamine oxidase inhibitory activity of Rhinocladiella sp. lgt-3,which was an endophytic fungus isolated from Tripterygium wilfordii.
Methods: Compounds were isolated from by various column chromatography. The structures were elucidated by various spectral data. The activity was tested by using 96-well micro-plated method.
Results: 10 compounds were isolated and identified as 3,4-dihydro-3,4,8-trihydroxy-1( 2H)-naphthalenone( 1),( 3R,4R)-4-hydroxymellein( 2),O-methylmellein( 3),4-( 2-hydroxybutynoxy) benzoic acid( 4),2,4-dihydroxy-6-(( R)-4-hydroxy-2-oxopentyl)-3-methylbenzaldehyde( 5),( Z)-N-( 4-hydroxystyryl) formamide( 6), aspterric acid( 7),bufotenin( 8),5-hydroxy-N,N-dimethyltryptamine-N-oxide( 9)and 5-methoxy-N,N-dimethyltryptamine( 10). Compounds 1,8 ~ 10 showed moderate anti-monoamine oxidase activity with IC50 values of 32,36,50,56 μmol/L, respectively.
Conclusion: Compounds 3 ~ 10 are isolated from Rhinocladiella genus for the first time and the anti-monoamine oxidase activity of compound 1 are reported for the first time.
目的:研究雷公藤内生真菌Rhinocladiella sp. lgt-3的化学成分及单胺氧化酶抑制活性。方法:采用各种柱层析法分离化合物。利用各种光谱数据对其结构进行了分析。采用96孔微镀法测定活性。结果:分离得到10个化合物，分别鉴定为3,4-二氢-3,4,8-三羟基-1(2H)-萘醌(1)、(3R,4R)-4-羟基甲氧基(2)、o-甲基甲氧基(3)、4-(2-羟基丁氧基)苯甲酸(4)、2,4-二羟基-6-((R)-4-羟基-2-氧戊基)-3-甲基苯甲醛(5)、(Z)- n -(4-羟基苯基)甲酰胺(6)、天冬氨酸(7)、丁烯酮(8)、5-羟基-n, n -二甲基色胺-n -氧化物(9)和5-甲氧基-n, n -二甲基色胺(10)。化合物1、8 ~ 10具有中等抗单胺氧化酶活性，IC50值分别为32、36、50、56 μmol/L。结论:化合物3 ~ 10为首次从该属植物中分离得到，化合物1抗单胺氧化酶活性为首次报道。
期刊介绍：
"Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.
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