[Sijunzi Decoction Promote the Repair of Intestinal Epithelial Cell Injury via Activating TLR-2/My D88 Signaling Pathway].

中药材 Pub Date : 2016-09-01

Hou-pan Song, Ru-yi Li, Hui-yong Huang, Xiong Cai, Zhen-yi Yuan, Ping-an Liu, Chi Zhou

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Abstract

Objective: To study the effect and mechanism of Sijunzi decoction( SJZD) containing serum on the repairing of gastrointestinal mucosal damage.

Methods: SJZD containing serum was prepared by serum pharmacological method. Cell migration model was established by tips scratch method, Real-time-cell-analyzer( RTCA) was used to mensurate IEC-6 cell proliferation, the mRNA and protein expression of TLR-2 and My D88 mRNA were detected by qRT-PCR and Western blot analysis,respectively.

Results: Medium dose( 10%) and high dose( 20%) of SJZD containing serum stimulated IEC-6 cell migration at 8 h after cell damage; medium dose( 10%)and high dose( 20%) of SJZD containing serum increased IEC-6 cell proliferation at 12 h after cell damage; low dose( 5%),medium dose( 10%) and high dose( 20%) of SJZD containing serum enhanced IEC-6 proliferation both at 24 h and 36 h after cell damage. Medium dose( 10%) and high dose( 20%) of SJZD containing serum upregulated TLR-2 and My D88 mRNA and protein expression,respectively.

Conclusion: Sijunzi decoction can repair the injury of gastrointestinal mucosal barrier,the mechanism may be related to its effect on activating TLR-2 / My D88 signaling pathway,and promoting IEC-6 cell migration and proliferation.

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[四君子汤通过激活TLR-2/My D88信号通路促进肠上皮细胞损伤修复]。

目的:研究含血清四君子汤对胃肠粘膜损伤的修复作用及其机制。方法:采用血清药理学方法制备含血清的SJZD。采用尖端划痕法建立细胞迁移模型，采用实时细胞分析仪(Real-time-cell-analyzer, RTCA)检测IEC-6细胞的增殖情况，分别采用qRT-PCR和Western blot检测TLR-2和My D88 mRNA的mRNA和蛋白表达。结果:中剂量(10%)和高剂量(20%)含血清的SJZD在细胞损伤后8 h刺激了IEC-6细胞的迁移;含血清的SJZD中剂量(10%)和高剂量(20%)在细胞损伤后12 h促进了IEC-6细胞的增殖;低剂量(5%)、中剂量(10%)和高剂量(20%)含血清的SJZD在细胞损伤后24 h和36 h均能增强IEC-6的增殖。中剂量(10%)和高剂量(20%)SJZD分别上调血清中TLR-2和My D88 mRNA和蛋白的表达。结论:四君子汤具有修复胃肠道粘膜屏障损伤的作用，其机制可能与其激活TLR-2 / My D88信号通路，促进IEC-6细胞迁移和增殖有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

中药材

自引率

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发文量

11364

期刊介绍： "Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.