Anti-CRISPRs: Protein Inhibitors of CRISPR-Cas Systems.

IF 12.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Annual review of biochemistry Pub Date : 2020-06-20 Epub Date: 2020-03-18 DOI:10.1146/annurev-biochem-011420-111224
Alan R Davidson, Wang-Ting Lu, Sabrina Y Stanley, Jingrui Wang, Marios Mejdani, Chantel N Trost, Brian T Hicks, Jooyoung Lee, Erik J Sontheimer
{"title":"Anti-CRISPRs: Protein Inhibitors of CRISPR-Cas Systems.","authors":"Alan R Davidson, Wang-Ting Lu, Sabrina Y Stanley, Jingrui Wang, Marios Mejdani, Chantel N Trost, Brian T Hicks, Jooyoung Lee, Erik J Sontheimer","doi":"10.1146/annurev-biochem-011420-111224","DOIUrl":null,"url":null,"abstract":"<p><p>Clustered regularly interspaced short palindromic repeats (CRISPR) together with their accompanying <i>cas</i> (CRISPR-associated) genes are found frequently in bacteria and archaea, serving to defend against invading foreign DNA, such as viral genomes. CRISPR-Cas systems provide a uniquely powerful defense because they can adapt to newly encountered genomes. The adaptive ability of these systems has been exploited, leading to their development as highly effective tools for genome editing. The widespread use of CRISPR-Cas systems has driven a need for methods to control their activity. This review focuses on anti-CRISPRs (Acrs), proteins produced by viruses and other mobile genetic elements that can potently inhibit CRISPR-Cas systems. Discovered in 2013, there are now 54 distinct families of these proteins described, and the functional mechanisms of more than a dozen have been characterized in molecular detail. The investigation of Acrs is leading to a variety of practical applications and is providing exciting new insight into the biology of CRISPR-Cas systems.</p>","PeriodicalId":7980,"journal":{"name":"Annual review of biochemistry","volume":"89 ","pages":"309-332"},"PeriodicalIF":12.1000,"publicationDate":"2020-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1146/annurev-biochem-011420-111224","citationCount":"76","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annual review of biochemistry","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1146/annurev-biochem-011420-111224","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2020/3/18 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 76

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR) together with their accompanying cas (CRISPR-associated) genes are found frequently in bacteria and archaea, serving to defend against invading foreign DNA, such as viral genomes. CRISPR-Cas systems provide a uniquely powerful defense because they can adapt to newly encountered genomes. The adaptive ability of these systems has been exploited, leading to their development as highly effective tools for genome editing. The widespread use of CRISPR-Cas systems has driven a need for methods to control their activity. This review focuses on anti-CRISPRs (Acrs), proteins produced by viruses and other mobile genetic elements that can potently inhibit CRISPR-Cas systems. Discovered in 2013, there are now 54 distinct families of these proteins described, and the functional mechanisms of more than a dozen have been characterized in molecular detail. The investigation of Acrs is leading to a variety of practical applications and is providing exciting new insight into the biology of CRISPR-Cas systems.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
抗 CRISPRs:CRISPR-Cas系统的蛋白质抑制剂。
细菌和古细菌中经常出现有规律间隔短回文重复序列(CRISPR)及其伴随的Cas(CRISPR相关)基因,用于抵御外来DNA(如病毒基因组)的入侵。CRISPR-Cas 系统能够适应新遇到的基因组,因此提供了一种独特而强大的防御能力。这些系统的适应能力已被开发利用,成为基因组编辑的高效工具。CRISPR-Cas 系统的广泛使用促使人们需要控制其活性的方法。本综述将重点介绍抗CRISPRs(Acrs),即由病毒和其他移动遗传因子产生的能有效抑制CRISPR-Cas系统的蛋白质。抗CRISPRs蛋白于2013年被发现,目前已有54个不同的家族被描述,其中十多个家族的功能机制已得到详细的分子表征。对Acrs的研究正在带来各种实际应用,并为CRISPR-Cas系统的生物学提供了令人兴奋的新见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Annual review of biochemistry
Annual review of biochemistry 生物-生化与分子生物学
CiteScore
33.90
自引率
0.00%
发文量
31
期刊介绍: The Annual Review of Biochemistry, in publication since 1932, sets the standard for review articles in biological chemistry and molecular biology. Since its inception, these volumes have served as an indispensable resource for both the practicing biochemist and students of biochemistry.
期刊最新文献
A Lipid-Raft Theory of Alzheimer's Disease. Lipid Quality Control and Ferroptosis: From Concept to Mechanism. A Life of Translocations. Signaling from RAS to RAF: The Molecules and Their Mechanisms. The Nicotinic Acetylcholine Receptor and Its Pentameric Homologs: Toward an Allosteric Mechanism of Signal Transduction at the Atomic Level.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1