Protection of human intestinal epithelial cells from oxidative stress caused by mercury using lactic acid bacteria.

IF 2.9 4区 医学 Q2 Agricultural and Biological Sciences Bioscience of Microbiota, Food and Health Pub Date : 2020-01-01 Epub Date: 2020-05-02 DOI:10.12938/bmfh.2019-049
Hideki Kinoshita, Mai Jumonji, Shin Yasuda, Keiji Igoshi
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引用次数: 4

Abstract

Heavy metals are harmful to human health. Therefore, we investigated the biosorption of heavy metals by lactic acid bacteria (LAB). Of all the tested heavy metals, biosorption by LAB was highest for mercury, followed by lead, cadmium, and finally arsenic. The viability of HCT-116 cells was reduced by half in the presence of 7.5 µg/mL mercury but recovered after the addition of selected LAB strains. HCT-116 cells showed increased superoxide dismutase and catalase activities, whereas glutathione peroxidase activities decreased significantly. Addition of Lactobacillus sakei TOKAI 57m recovered all antioxidant enzyme activities. Our results suggest that this strain can be used for cellular detoxification.

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利用乳酸菌保护人体肠上皮细胞免受汞引起的氧化应激。
重金属对人体健康有害。因此,我们研究了乳酸菌(LAB)对重金属的生物吸附。在所有被测试的重金属中,乳酸菌对汞的生物吸附性最高,其次是铅、镉,最后是砷。在7.5µg/mL汞的作用下,HCT-116细胞的活力降低一半,但在加入选定的LAB菌株后恢复。HCT-116细胞超氧化物歧化酶和过氧化氢酶活性升高,而谷胱甘肽过氧化物酶活性显著降低。添加清井乳杆菌TOKAI 57m后,抗氧化酶活性恢复。我们的结果表明,该菌株可用于细胞解毒。
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来源期刊
Bioscience of Microbiota, Food and Health
Bioscience of Microbiota, Food and Health Immunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
5.50
自引率
3.20%
发文量
24
期刊介绍: Bioscience of Microbiota, Food and Health (BMFH) is a peer-reviewed scientific journal with a specific area of focus: intestinal microbiota of human and animals, lactic acid bacteria (LAB) and food immunology and food function. BMFH contains Full papers, Notes, Reviews and Letters to the editor in all areas dealing with intestinal microbiota, LAB and food immunology and food function. BMFH takes a multidisciplinary approach and focuses on a broad spectrum of issues.
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