{"title":"Electrophysiological recordings from the neuromuscular junction of C. elegans.","authors":"Janet E Richmond","doi":"10.1895/wormbook.1.112.1","DOIUrl":null,"url":null,"abstract":"<p><p>Electrophysiology provides a quantifiable measure of synaptic activity useful in the functional analysis of synaptic proteins. Recent advances in the application of this technique to C. elegans provides a means of coupling genetics to electrophysiological analysis, providing new insights into the molecular mechanisms regulating neurotransmission. Here we describe a dissection technique that exposes the neuromuscular junctions of C. elegans for electrophysiological analysis. This technique can be adapted to record from virtually any excitable cell in the worm.</p>","PeriodicalId":75344,"journal":{"name":"WormBook : the online review of C. elegans biology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2006-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4780886/pdf/","citationCount":"36","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"WormBook : the online review of C. elegans biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1895/wormbook.1.112.1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 36
Abstract
Electrophysiology provides a quantifiable measure of synaptic activity useful in the functional analysis of synaptic proteins. Recent advances in the application of this technique to C. elegans provides a means of coupling genetics to electrophysiological analysis, providing new insights into the molecular mechanisms regulating neurotransmission. Here we describe a dissection technique that exposes the neuromuscular junctions of C. elegans for electrophysiological analysis. This technique can be adapted to record from virtually any excitable cell in the worm.