NtMYB12 requires for competition between flavonol and (pro)anthocyanin biosynthesis in Narcissus tazetta tepals.

IF 10.6 Q1 HORTICULTURE Molecular Horticulture Pub Date : 2023-02-08 DOI:10.1186/s43897-023-00050-7
Jingwen Yang, Xi Wu, Cristina Belen Aucapiña, Deyu Zhang, Jiazhi Huang, Ziyuan Hao, Yu Zhang, Yujun Ren, Ying Miao
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引用次数: 4

Abstract

The color of flowers is one of the main characteristics adopted for plants to attract pollinators to ensure the reproductive success of the plant, they are also important in their ornamental appeal in Narcissus plant. In this study, we identified a NtMYB12 locus encoding an R2R3-MYB transcription factor. Comparative transcriptome analysis of loss- and gain- of NtMYB12 tissue relative to wild-type narcissus showed NtMYB12 was mainly involved in flavonol and phenylpropanoid metabolic pathways. Biochemical evidences of dual-luciferase activity and chromatin immunoprecipitation assay supported that MYB12 directly bound to promoters of NtFLS, NtLAR, and NtDFR that were cloned by genome walking assay, and activated NtFLS and NtLAR expression but repressed NtDFR expression. More interestingly, NtMYB12 can interact with NtbHLH1 and NtWD40-1 proteins via R3 domain that were selected by transcriptome-based WGCNA and confirmed by yeast two hybrid, bimolecular fluorescence complementation and coimmunoprecipitation assay. Interaction of NtMYB12 with NtbHLH1 and NtWD40-1 forming MYB-bHLH-WD40 triplex specially activated NtDFR and NtANS expression and promoted (pro)anthocyanin accumulation, while NtMYB12 alone activated NtFLS and NtLAR expression and accumulated flavonols, but repressed NtDFR expression. These results indicated that NtMYB12 alone or NtMYB12-bHLH1-WD40-1 triplex requires for competition of metabolism fluxes between flavonol and (pro)anthocyanin biosynthesis. NtMYB12 dually functions on flavonol and proanthocyanin biogenesis via physically binding to NtFLS and NtLAR promoter activating their expression and on (pro)anthocyanin biosynthesis via NtMYB12-NtWD40-NtbHLH (MBW) triplex activating NtDFR and NtANS expression. Requirement of NtMYB12 alone or MBW complex for the competition between flavonol and anthocyanin biosynthesis results in narcissus colorized petal traits.

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NtMYB12需要水仙中黄酮醇和(原)花青素生物合成之间的竞争。
花的颜色是植物吸引传粉者以确保植物繁殖成功的主要特征之一,它们在水仙植物中的观赏吸引力也很重要。在这项研究中,我们鉴定了一个编码R2R3-MYB转录因子的NtMYB12基因座。NtMYB12组织相对于野生型水仙的损失和获得的比较转录组分析表明,NtMYB12主要参与黄酮醇和苯丙烷代谢途径。双荧光素酶活性和染色质免疫沉淀分析的生化证据支持MYB12直接与基因组行走分析克隆的NtFLS、NtLAR和NtDFR启动子结合,并激活NtFLS和NtLAR的表达,但抑制NtDFR的表达。更有趣的是,NtMYB12可以通过基于转录组的WGCNA选择的R3结构域与NtbHLH1和NtWD40-1蛋白相互作用,并通过酵母双杂交、双分子荧光互补和共免疫沉淀分析证实。NtMYB12与NtbHLH1和NtWD40-1的相互作用形成MYB-bHLH-WD40三链,特别激活NtDFR和NtANS的表达并促进(原)花青素的积累,而NtMYB12单独激活NtFLS和NtLAR的表达并积累黄酮醇,但抑制NtDFR的表达。这些结果表明,单独的NtMYB12或NtMYB12-bHLH1-WD40-1三链体需要黄酮醇和(原)花青素生物合成之间的代谢通量竞争。NtMYB12通过与NtFLS和NtLAR启动子物理结合激活其表达,对黄酮醇和原花青素的生物合成具有双重作用,并通过NtMYB12-NtWD40-NtbHLH(MBW)三链激活NtDFR和NtANS表达,对(原)花青素的生物合成具有双重作用。单独的NtMYB12或MBW复合物对黄酮醇和花青素生物合成之间的竞争的需求导致水仙的花瓣着色特征。
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来源期刊
Molecular Horticulture
Molecular Horticulture horticultural research-
CiteScore
8.00
自引率
0.00%
发文量
24
审稿时长
12 weeks
期刊介绍: Aims Molecular Horticulture aims to publish research and review articles that significantly advance our knowledge in understanding how the horticultural crops or their parts operate mechanistically. Articles should have profound impacts not only in terms of high citation number or the like, but more importantly on the direction of the horticultural research field. Scope Molecular Horticulture publishes original Research Articles, Letters, and Reviews on novel discoveries on the following, but not limited to, aspects of horticultural plants (including medicinal plants): ▪ Developmental and evolutionary biology ▪ Physiology, biochemistry and cell biology ▪ Plant-microbe and plant-environment interactions ▪ Genetics and epigenetics ▪ Molecular breeding and biotechnology ▪ Secondary metabolism and synthetic biology ▪ Multi-omics dealing with data sets of genome, transcriptome, proteome, metabolome, epigenome and/or microbiome. The journal also welcomes research articles using model plants that reveal mechanisms and/or principles readily applicable to horticultural plants, translational research articles involving application of basic knowledge (including those of model plants) to the horticultural crops, novel Methods and Resources of broad interest. In addition, the journal publishes Editorial, News and View, and Commentary and Perspective on current, significant events and topics in global horticultural fields with international interests.
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