Evaluation of Protective Effect of Ajwa Seed And Fruit on Renal Histopathological Changes in Diabetic Nephropathic Rats

Esculapio Pub Date : 2021-03-31 DOI:10.51273/ESC21.2517121

I. Imran, Maryam Mansoor, F. Naqvi, M. Akhtar, W. Arshad, Faiza Khan

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Abstract

Objective: To investigate the protective effect of Ajwa date seed and fruit on renal histological changes in alloxan induced diabetic rats. Methods: This was an experimental study and was conducted in Post Graduate Medical Institute. The duration of study was 6 weeks. In this study random allotment of 32 rats was done in four groups. Group 1 was treated as control. Diabetes was induced in the 2nd, 3rd and 4th group by alloxan injection intra peritoneally. Group 2 was diabetic non treated while group 3 and 4 were treated with Ajwa seed and flesh respectively. After six weeks, animals were anaesthetized and kidneys were then removed without delay and weighed. Kidney paraffin sections were prepared and stained with hematoxylin& eosin (H&E) and with Periodic acid Schiff (PAS) technique. Glomerular diameters were estimated. Glomerular volume determined by stage micrometer. Vascular, tubular injury and glomerular sclerosis were studied semi quantitatively. Results: The data showed that Ajwa date seed significantly reduced hyperglycemia but did not normalize the fasting blood glucose. We found exceedingly significant improvement in kidney weight, glomerular diameter, tubular and vascular injury with Ajwa date seed suggesting reduction in diabetic nephropathy. Ajwa seed diet found more effective in reducing nephropathy than Ajwa fruit diet. Current study displayed that the seed of Ajwa showed significant improvement in renal histological characteristics in diabetic rats. Conclusion: The findings showed that Ajwa date seed and flesh reduce loss of tubular and vascular damage in alloxan induced diabetes. Key Words: Oxidative stress, Kidney, Diabetes, Ajwa, Antioxidant, histopathology How to cite: Imran I., Mansoor M., Naqvi F., Akhtar M., Arshad W., Khan F. Evaluation of protective effect of Ajwa seed and fruit on renal histopathological changes in diabetic nephropathic rats. Esculapio 2021;17(01):104-109

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Ajwa种子和果实对糖尿病肾病大鼠肾脏组织病理变化的保护作用评价

目的：研究阿胶枣仁和果实对四氧嘧啶糖尿病大鼠肾脏组织学变化的保护作用。方法：本研究在研究生院进行。研究持续时间为6周。本研究将32只大鼠随机分为四组。第1组为对照组。第2组、第3组和第4组通过腹膜内注射四氧嘧啶诱发糖尿病。第2组为糖尿病非治疗组，第3组和第4组分别用Ajwa籽和肉治疗。六周后，对动物进行麻醉，然后立即取出肾脏并称重。制备肾脏石蜡切片，并用苏木精和伊红（H&E）和碘酸希夫（PAS）技术进行染色。估计肾小球直径。肾小球体积由载物台测微计测定。对血管、肾小管损伤和肾小球硬化进行了半定量研究。结果：实验结果表明，冬枣仁能显著降低高血糖，但不能使空腹血糖正常化。我们发现Ajwa椰枣籽在肾脏重量、肾小球直径、肾小管和血管损伤方面有非常显著的改善，这表明糖尿病肾病的发病率降低。Ajwa种子饮食在减少肾病方面比Ajwa水果饮食更有效。目前的研究表明，Ajwa的种子对糖尿病大鼠的肾脏组织学特征有显著改善。结论：Ajwa枣籽和果肉可减少四氧嘧啶诱导的糖尿病患者的肾小管和血管损伤。关键词：氧化应激，肾脏，糖尿病，Ajwa，抗氧化剂，组织病理学如何引用：Imran I.，Mansoor M.，Naqvi F.，Akhtar M.，Arshad W.，Khan F.评价Ajwa种子和水果对糖尿病肾病大鼠肾脏组织病理学变化的保护作用。Esculapio 2021；17（01）：104-109

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Esculapio

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12 weeks