Analysis of Immune Profiles Related to Disease Severity in COVID-19 by Flow Cytometry

IF 0.5 4区 医学 Q4 MICROBIOLOGY Jundishapur Journal of Microbiology Pub Date : 2023-08-17 DOI:10.5812/jjm-138835
Tuğrul Hoşbul, S. Oren, C. Artuk, C. Aydoğan, İrem Unat, S. Şenkal, Gamze İçen, Tugba Fatsa, R. Gumral, F. Şahiner, M. Kızılgun, M. Yavuz
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Abstract

Background: More than 768 million people have been affected by COVID-19. Identifying lymphocyte subsets and cytokine level abnormalities in COVID-19 patients is essential to gain new insights and data on immunity mechanisms against viral infections. Objectives: We used flow cytometry to determine the relationship between disease severity, lymphocyte subsets distribution, and cytokine level alterations in COVID-19 patients. Methods: Totally 94 COVID-19 patients (32 mild, 31 moderate, and 31 severe) and 27 healthy individuals were included in the cross-sectional study. The distribution of peripheral lymphocyte subsets and cytokine levels was assessed by flow cytometry. Results: The percentages of CD56+ Natural Killer (NK) cells in all patient groups and total T lymphocytes in moderate and severe groups were significantly lower than those in the control group (P < 0.001). Also, IL-2 (P < 0.001), IL-17A (P < 0.001), IL-4 (P < 0.001), IL-6 (P < 0.001), TNF-α (P = 0.004), IP-10 (P < 0.001), IFN-λ1 (IL-29) (P < 0.001), IFN-λ2/3 (IL-28A/B) (P = 0.011), IFN-β (P < 0.001), IL-10 (P < 0.001), and IFN-γ (P < 0.001) levels were statistically higher in patients than in the controls. Conclusions: Our data revealed that increased levels of certain cytokines in peripheral blood contribute to disease severity. Increased CRP (OR: 1.012, %95 CI: 1.002 - 1.023, P = 0.038) and IL-10 (OR: 1.068, %95 CI: 1.000 - 1.141, P = 0.049) levels, decreased CD56+ NK percentage (OR: 0.576, %95 CI: 0.376 - 0.882, P = 0.011) and lymphocyte count (OR: 0.02, %95 CI: 0.001 - 0.368, P = 0.009), and the presence of diabetes mellitus and mechanical ventilation were independent predictors of mortality.
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流式细胞术分析新冠肺炎患者与疾病严重程度相关的免疫谱
背景:超过7.68亿人受到新冠肺炎的影响。识别新冠肺炎患者的淋巴细胞亚群和细胞因子水平异常对于获得有关病毒感染免疫机制的新见解和数据至关重要。目的:我们使用流式细胞术来确定新冠肺炎患者疾病严重程度、淋巴细胞亚群分布和细胞因子水平变化之间的关系。方法:共有94名新冠肺炎患者(32名轻度、31名中度和31名重度)和27名健康人参加横断面研究。通过流式细胞术评估外周淋巴细胞亚群的分布和细胞因子水平。结果:CD56+自然杀伤(NK)细胞在所有患者组中的百分比和中重度组的总T淋巴细胞的百分比均显著低于对照组(P<0.001)。此外,IL-2(P<0.01)、IL-17A(P<001)、IL-4(P<0.05)、IL-6(P<0.005)、TNF-α(P=0.004)、IP-10(P<.001)、IFN-λ1(IL-29)、IFNλ2/3(IL-28A/B)(P=0.011),患者的IFN-β(P<0.001)、IL-10(P<0.01)和IFN-γ(P<001)水平在统计学上高于对照组。结论:我们的数据显示,外周血中某些细胞因子水平的升高会导致疾病的严重程度。CRP(OR:1.012,95%CI:1.002-1.023,P=0.038)和IL-10(OR:1.068,95%CI:1.000-1.141,P=0.049)水平升高,CD56+NK百分比(OR:0.576,95%CI:0.376-0.882,P=0.011)和淋巴细胞计数(OR:0.02,95%CI:0.001-0.368,P=0.009)降低,以及糖尿病和机械通气的存在是死亡率的独立预测因素。
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来源期刊
CiteScore
1.30
自引率
0.00%
发文量
56
审稿时长
6-12 weeks
期刊介绍: Jundishapur Journal of Microbiology, (JJM) is the official scientific Monthly publication of Ahvaz Jundishapur University of Medical Sciences. JJM is dedicated to the publication of manuscripts on topics concerning all aspects of microbiology. The topics include medical, veterinary and environmental microbiology, molecular investigations and infectious diseases. Aspects of immunology and epidemiology of infectious diseases are also considered.
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