{"title":"Expression of triggering receptor expressed on myeloid cells in periventricular leukomalacia of neonatal rat model","authors":"Da-yu Wang, Xiao-yan Ye, Yan Xu","doi":"10.3760/CMA.J.CN371468-20191213-00912","DOIUrl":null,"url":null,"abstract":"Objective \nTo investigate the expression of triggering receptor expressed on myeloid cells(TREMs)in periventricular leukomalacia(PVL) of the neonatal rat model. \n \n \nMethods \nThirty-two 3-day-old neonatal rats were double-blinded randomly divided into Sham group and Model group.The PVL rat model was established by ligating right carotid artery and oxygen deprivation.Hematoxylin-eosin (HE) was adopted to compare pathological changes of brain tissue between the two groups, and immunofluorescence was adopted to detect the expression of myline basic protein (MBP) in the right hemisphere of the two groups.Western blot was performed to detect the expression of TREM1 and TREM2 in the right hemisphere of the two groups. \n \n \nResults \nThe results of HE staining showed that the brain tissues of Model group were significantly damaged compared with that of Sham group, and the mean fluorescence intensity of MBP in Model group(26.629±2.317) was significantly lower than that in Sham group(33.579±2.824), with statistically significant differences(t=9.124, P<0.05). The expression of TREM1 in Model group(0.789 ±0.120) was higher than that in Sham group(0.567±0.093), with statistically significant differences(t=-3.891, P<0.05). The expression of TREM2 in Model group(0.544±0.133) was lower than that in Sham group(0.791±0.118), with statistically significant differences(t=3.667, P<0.05). \n \n \nConclusion \nThe expressions of TREM1 and TREM2 in the neonatal rat model of PVL change abnormally, suggesting that TREMs may be involved in the pathological process of preterm white matter injury. \n \n \nKey words: \nPeriventricular leukomalacia; White matter injury; Brain injury; Triggering receptor expressed on myeloid cells; Rat","PeriodicalId":9940,"journal":{"name":"中华行为医学与脑科学杂志","volume":"29 1","pages":"109-113"},"PeriodicalIF":0.0000,"publicationDate":"2020-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华行为医学与脑科学杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3760/CMA.J.CN371468-20191213-00912","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective
To investigate the expression of triggering receptor expressed on myeloid cells(TREMs)in periventricular leukomalacia(PVL) of the neonatal rat model.
Methods
Thirty-two 3-day-old neonatal rats were double-blinded randomly divided into Sham group and Model group.The PVL rat model was established by ligating right carotid artery and oxygen deprivation.Hematoxylin-eosin (HE) was adopted to compare pathological changes of brain tissue between the two groups, and immunofluorescence was adopted to detect the expression of myline basic protein (MBP) in the right hemisphere of the two groups.Western blot was performed to detect the expression of TREM1 and TREM2 in the right hemisphere of the two groups.
Results
The results of HE staining showed that the brain tissues of Model group were significantly damaged compared with that of Sham group, and the mean fluorescence intensity of MBP in Model group(26.629±2.317) was significantly lower than that in Sham group(33.579±2.824), with statistically significant differences(t=9.124, P<0.05). The expression of TREM1 in Model group(0.789 ±0.120) was higher than that in Sham group(0.567±0.093), with statistically significant differences(t=-3.891, P<0.05). The expression of TREM2 in Model group(0.544±0.133) was lower than that in Sham group(0.791±0.118), with statistically significant differences(t=3.667, P<0.05).
Conclusion
The expressions of TREM1 and TREM2 in the neonatal rat model of PVL change abnormally, suggesting that TREMs may be involved in the pathological process of preterm white matter injury.
Key words:
Periventricular leukomalacia; White matter injury; Brain injury; Triggering receptor expressed on myeloid cells; Rat
期刊介绍：
"Chinese Journal of Behavioral Medicine and Brain Science" (CN 37-1468/R, ISSN 1674-6554) is a national academic journal under the supervision of the National Health Commission, sponsored by the Chinese Medical Association and Jining Medical College. The journal was founded in June 1992 and was formerly known as "Chinese Journal of Behavioral Medicine" (1992-1993) and "Chinese Behavioral Medical Science" (1994-2008). In 2009, it was renamed "Chinese Journal of Behavioral Medicine and Brain Science" with the approval of the State Administration of Press, Publication, Radio, Film and Television.
The purpose of "Chinese Journal of Behavioral Medicine and Brain Science" is to implement the health and health policies of the Party and the State, implement the principle of combining theory with practice and popularization and improvement, and reflect the major progress in the theory and practical application of behavioral medicine and brain science in my country. It publishes academic papers and scientific research results in the field of behavioral medicine and brain science in my country, and has columns such as monographs/reviews, basic research, clinical research, health prevention, methods and techniques, psychological behavior and evaluation, and systematic evaluation.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
