Investigation of Apoptotic Effect of Propofol, Dexmedetomidine and Medetomidine on Oocyte Cumulus Granulosa Cells in Rats

Q4 Medicine Anestezi Dergisi Pub Date : 2023-01-30 DOI:10.54875/jarss.2023.88942

A. Tas Tuna, H. Kocayiğit, Gürkan Demir, Ozcan Budak, M. S. Bostancı, H. Cakiroglu, D. B. Gunaydin

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Abstract

Objective: We aimed to evaluate the potential apoptotic effects of propofol, dexmedetomidine and medetomidine that were used during oocyte retrieval on cumulus cells in rat ovulation induction model. Methods: After the ovulation induction model was created, rats were received dexmedetomidine in Group D, propofol in Group P, medetomidine in Group M. Oocytes collection was performed 10 minutes after the administration of study drugs. For Caspase-3 immunohistochemical evaluation, the staining level was scored in five randomly selected areas and the areas with the highest score were determined. Immunohistochemical staining scoring for each section was performed using a scoring algorithm called H-score. Results: Caspase-3 expression in cumulus cells was found to be lowest in Group D and highest in Group P. The mean caspase-3 H-score was lower in Group D than that of Group P and M (p<0.001), and in Group M than that of Group P (p<0.001). Conclusion: Our results demonstrate that dexmedetomidine and medetomidine exhibit less apoptotic effects in terms of caspase-3 activity in oocyte cumulus cells than propofol in a rat ovulation induction model. Keywords: Apoptosis, cumulus cells, dexmedetomidine, medetomidine, propofol, oocyte retrieval

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异丙酚、右美托咪定、美托咪定对大鼠卵丘颗粒细胞凋亡影响的研究

目的：评价丙泊酚、右美托咪定和美托咪啶在取卵过程中对大鼠促排卵模型卵丘细胞的潜在凋亡作用。方法：建立大鼠促排卵模型后，D组给予右美托咪定，P组给予丙泊酚，M组给予美托咪啶。给药10分钟后收集卵母细胞。对于Caspase-3免疫组织化学评价，在随机选择的五个区域中对染色水平进行评分，并确定得分最高的区域。使用称为H-核心的评分算法对每个切片进行免疫组织化学染色评分。结果：D组卵丘细胞Caspase-3表达最低，P组最高，结论：在大鼠排卵诱导模型中，右美托咪定和美托咪啶对卵母细胞卵丘细胞胱天蛋白酶-3活性的凋亡作用小于丙泊酚。关键词：细胞凋亡，卵丘细胞，右美托咪定，美托咪啶，丙泊酚，取卵

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Anestezi Dergisi Medicine-Anesthesiology and Pain Medicine

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