Antiviral Properties of Cerium Nanoparticles

Abstract

According to the results of the study, the threshold limit values (TLVs) of CeO 2 , and Ce nanoparticles (NPs) for the culture of swine kidney embryonic cell line (SKECL) have been estimated. The TLVs are 0.1 μg/cm 3 for Ce NPs, and 1 μg/cm 3 for CeO 2 NPs. All NPs were non-toxic for white mice at concentration of 2000 mg/kg, which opens up a perspective for the further use of these NPs in the development of antiviral substances. CeO 2 , and Ce NPs have decreased the titer of Teschovirus by 1.46–2 lg 10 TCD 50 / cm 3 at TLVs at all stages of virus reproduction. Sensitivity of the strain Dniprovskyi-34 of species Teschovirus A, serotype Porcine teschovirus 1 (PTV-1), to solvents, proteolytic enzyme trypsin under the presence of CeO 2 , and Ce NPs has been studied. Under the presence of solvents, and trypsin, NPs decreased the infectious titer of the virus slightly by only 0.23 lg 10 TCD 50 /cm 3 . CeO 2 , and Ce NPs have not shown antiviral activity under heating to 50 ° C, either with the addition of 1 M solution of MgCl 2 or without it, and did not cause significant decrease of infectious activity of the virus under pH values 2.0, 7.2, and 11.0 within 10 minutes exposition. Ability of CeO 2 , and Ce NPs to interact with viral particles, change their morphology, which can affect infective activity of the virus significantly, has been shown. Addition of CeO 2 , and Ce NPs to the antigen has caused the decrease of the titer of virus-neutralizing antibodies, though it remained high. The results of the studies can be used in development of disinfectants, and antiviral drugs. strain of Teschovirus of Porcine teschovirus -1 serotype under the influence of CeO 2 , and Ce NPs according to prophylactic, therapeutic, and virucidal plots been studied in experiments using SKECL cell culture. It was found that according to the prophylactic plot CeO 2 , and Ce NPs have caused a decrease in the virus titer by 2.0 lg TCD 50 /cm 3 , according to the treatment plot - by 1.5 lg TCD 50 /cm 3 . CeO 2 and Ce NPs have reduced the virus titer by 1.46, and 2.0 lg TCD 50 /cm 3 , respectively, according to virucidal plot.