RAPD Profiles of Rhynchostylis gigantea (Lindl.) Ridl. Collected from Puspa Nirmala Orchids Banyumas, Central Java

Q3 Chemistry Molekul Pub Date : 2022-07-21 DOI:10.20884/1.jm.2022.17.2.6206
A. Susanto, Ali Romadhoni, M. Dwiati
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Abstract

Rhynchostylis gigantea (Lindl.) Ridl. is an orchid species spread over Southeast Asia countries. This species is very popular among ornamental plant collectors, especially due to its densely pack inflorescences. Hence, it is commercially found in many ornamental plant nursuries, such as Puspa Nirmala Orchids Banyumas, Central Java. Further development of the species should be supported by scientific data, particularly regarding the genetic variation. One of the molecular markers commonly used to study genetic variation is Random Amplified Polymorphic DNA (RAPD). This study aims to assess genetic variation of R. gigantea cultivars of Puspa Nirmala Orchids Banyumas collection  by RAPD profiles. Genomic DNAs were extracted from leaf samples of eight R. gigantea individuals, while RAPD markers were amplified using five random primers (OPA-15, OPK-16, OPP-15, OPP-08 and OPO-08). Descriptive analysis was employed on the data obtained. It was revealed that all of the primers resulted in a 100% monomorphism. This indicates an extremely low genetic variation among R. gigantea population of Puspa Nirmala Orchids collection, which is probably due to the same origin from a selected hybrid of the same crosses.
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Rhynchostylis gigantea(Lindl.)Ridl的RAPD图谱。收藏于中爪哇岛的Puspa Nirmala Orchids Banyumas
Rhynchostylis gigantea(林)Ridl。是一种分布在东南亚国家的兰花。该物种在观赏植物收藏家中非常受欢迎,尤其是由于其密集的花序。因此,它在许多观赏植物苗圃中都有商业价值,如爪哇中部的Puspa Nirmala兰花Banyumas。该物种的进一步发展应得到科学数据的支持,特别是关于遗传变异的数据。随机扩增多态DNA(RAPD)是研究遗传变异的常用分子标记之一。本研究旨在利用RAPD图谱评估Puspa Nirmala Orchids Banyumas收藏的R.gigantea品种的遗传变异。从8个巨型R.gigantea个体的叶片样本中提取基因组DNA,并使用5个随机引物(OPA-15、OPK-16、OPP-15、OPP-08和OPO-08)扩增RAPD标记。对获得的数据进行描述性分析。结果表明,所有引物都产生了100%的单态性。这表明Puspa Nirmala兰花收藏的R.gigantea种群的遗传变异极低,这可能是由于相同杂交种的相同起源。
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来源期刊
Molekul
Molekul Chemistry-Chemistry (all)
CiteScore
1.30
自引率
0.00%
发文量
31
审稿时长
4 weeks
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