Remarkable improvement of levansucrase production from a newly isolated Aspergillus niger MK788296 strain using agro-industrial wastes through statistical optimization techniques

IF 0.7 Q4 PHARMACOLOGY & PHARMACY Egyptian Pharmaceutical Journal Pub Date : 2023-01-01 DOI:10.4103/epj.epj_92_22
Nermeen Elzairy, F. Mostafa, W. A. Wahab, M. Abdel-Naby, Yasser M Ragab, A. Hashem
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Abstract

Background and objective Microbial levansucrase (LS) is a good source for the production of biologically active fructo-oligosaccharides and levan, which have diverse applications in pharmaceutical industries. Therefore, recent studies have focused on the enhancement of LS production through searching for potent microbial producers and optimization of the fermentation conditions. The present study aimed to use agro-industrial waste as a cost-effective carbon source for LS production and maximize the enzyme yield by optimization of the cultural conditions. Materials and methods A potent fungal producer of LS was isolated from an Egyptian soil sample that was collected from Giza Governorate at a depth of 5 cm and identified based on internal transcribed spacer identification and then submitted to the gene bank database. The production of LS by the isolated strain was optimized by evaluating the best fermentation state and agro-industrial waste to be used in the fermentation process. After that, further optimization of culture medium composition was established by two statistical designs: the Plackett–Burman design followed by central composite design. Results and conclusion The isolated strain was identified as Aspergillus niger MK788296. The first optimization approach declared that using the submerged fermentation technique and utilizing potato peels as the main carbon source led to a 2.4-fold increase in LS production. The statistical optimization resulted in a massive LS production (18870.3 U/ml) with a 59.4-fold increase in enzyme yield than the nonoptimized culture conditions.
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利用统计优化技术显著提高黑曲霉MK788296菌株利用农用工业废弃物生产蔗糖酶的效果
背景与目的微生物葡糖酶(LS)是生产具有生物活性的低聚果糖和葡糖的良好来源,在制药工业中有着广泛的应用。因此,最近的研究集中在通过寻找有效的微生物生产者和优化发酵条件来提高LS的产量。本研究旨在利用农业工业废物作为LS生产的成本效益高的碳源,并通过优化培养条件最大限度地提高酶产量。材料和方法从埃及吉萨省采集的深度为5 cm,并基于内部转录的间隔区鉴定进行鉴定,然后提交到基因库数据库。通过评估最佳发酵状态和发酵过程中使用的农业工业废物,对分离菌株生产LS进行了优化。之后,通过两种统计设计建立了培养基组成的进一步优化:Plackett–Burman设计和中央复合设计。结果与结论分离菌株经鉴定为黑曲霉MK788296。第一种优化方法表明,使用深层发酵技术并利用土豆皮作为主要碳源,LS产量增加了2.4倍。统计优化导致了大规模的LS生产(18870.3 U/ml),酶产量比未优化的培养条件增加59.4倍。
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来源期刊
Egyptian Pharmaceutical Journal
Egyptian Pharmaceutical Journal PHARMACOLOGY & PHARMACY-
CiteScore
1.10
自引率
0.00%
发文量
37
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