Transposon Mutagenesis and Analysis of Mutants in UniformMu Maize (Zea mays)

Q1 Agricultural and Biological Sciences Current protocols in plant biology Pub Date : 2016-09-01 DOI:10.1002/cppb.20029

Peng Liu, Donald R. McCarty, Karen E. Koch

引用次数: 19

Abstract

A wealth of maize mutants is now available with known sites of transposon insertions in over 45% of maize genes. Materials can be obtained free of charge from the UniformMu public resource through MaizeGDB.org or directly from the Maize Genetics COOP Stock Center. Specific mutants can be sought online based on gene-sequences of interest. A key feature of the UniformMu resource is the uniformity of wild-type controls, which facilitates characterization of mutant phenotypes. Methods developed for construction (transposon mutagenesis), analysis and utilization of the resource are described here. These include the high-throughput Mu-seq genotyping protocol that enables both forward and reverse approaches for linking genotypes-to-phenotypes. © 2016 by John Wiley & Sons, Inc.

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匀穆玉米转座子诱变及其突变体分析

现在有大量的玉米突变体，在超过45%的玉米基因中有已知的转座子插入位点。资料可以通过MaizeGDB.org从UniformMu公共资源免费获得，也可以直接从玉米遗传COOP库存中心获得。可以根据感兴趣的基因序列在网上寻找特定的突变体。UniformMu资源的一个关键特征是野生型对照的一致性，这有利于突变表型的表征。本文介绍了该资源的构建方法(转座子诱变)、分析和利用。其中包括高通量Mu-seq基因分型方案，该方案支持将基因型与表型连接起来的正向和反向方法。©2016 by John Wiley &儿子,Inc。

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Current protocols in plant biology Agricultural and Biological Sciences-Plant Science

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. Current Protocols in Plant Biology provides reproducible step-by-step instructions for protocols relevant to plant research. Furthermore, Current Protocols content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols in Plant Biology to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Plant Biology is the comprehensive source for protocols in the multidisciplinary field of plant biology, providing an extensive range of protocols from basic to cutting edge. Coverage includes: Extraction and analysis of DNA, RNA, proteins Chromosome analysis Transcriptional analysis Protein expression Metabolites Plant enzymology Epigenetics Plant genetic transformation Mutagenesis Arabidopsis, Maize, Poplar, Rice, and Soybean, and more.