Antioxidant and antibacterial activities of leaves crude extracts of Adenium obesum grown in Oman National Botanical Garden

Abstract

Globally, researchers are investigated and recommend that about two-thirds of the plant and marine species available have significant antioxidant and antibacterial activities. Plant reduce oxidative stress in cells and infections therefore they are useful to treat most common diseases eg., cancer, cardiovascular, inflammatory and various infectious diseases. The focus of this study is to prepare various extracts of Adenium obesum (A. obesum) that was collected from Oman National Botanical Garden, Muscat and evaluate antioxidant and antibacterial activities of the extracts using a modified established method. The different polarities leave crude extracts were prepared from dry leaf powder of A. obesum and fractioned with increasing solvent polarities starting from non-polar hexane solvent. The extracts from leaves with various polarities were used to estimate their antioxidant and antibacterial activities through 2,2-diphenyl-1-picrylhydrazyl (DPPH) and asgar disc bioassays. The maximum antioxidant and antibacterial activities were detected in ethyl acetate extracts with an inhibition of 88.27% and 25 mm and the chloroform extract showed the smallest activity with an inhibition of 2.57% and 5 mm. Based on experimental results, systematic order of antioxidant activity was ethyl acetate > butanol > water > hexane > methanol > chloroform extract and the order of antibacterial activity was ethyl acetate > methanol > butanol > water > hexane > chloroform extract. The findings demonstrated that the maximum activity plant leaves extract could form a good source for the isolation of diseases guided chemical compounds and further evaluation of their biological and pharmacological activities.