Cascade Signal Amplification Rapid Detection of Two Pathogenic Bacteria in Dairy Products Using Magnetic Bead Nanoprobes Combined with Immunochromatographic

Abstract

Enterobacter sakazakii and Staphylococcus aureus are common detection items with high pathogenicity in dairy products. In this study, we first achieved effective enrichment of target bacteria through immunomagnetic separation, realized signal amplification and sensitive detection of Enterobacter sakazakii and Staphylococcus aureus through quantum dots and biotin streptavidin system combined with immunochromatography. By optimizing the overall research, both bacterial detection sensitivities can reach 2 × 10 3 CFU/mL, which increases the detection sensitivity by nearly 2000 times compared to traditional immunochromatography. It can achieve simultaneous sensitive detection of foodborne pathogens, providing a new approach for food safety to quickly, sensitively, and accurately detect large molecular targets.