Effects of Different Quick-Freezing Methods and Storage Temperatures on the Content of Cryoprecipitated Blood Coagulation Factors in Fresh Plasma

Abstract

To observe the effect of different quick-freezing methods and different storage temperatures on the content of cryoprecipitated blood coagulation factors in fresh plasma. The convenient sampling method was used to select 200 ml of whole blood from 60 unpaid blood donors who entered the blood station from January 2022 to October 2022. The acquisition time is controlled within 10 min. The plasma samples were divided into six groups (A, B, C, D, E, F) by using a random number table, 10 in each group. Group A (plate freezer for 30 min, - 30 ℃ storage), Group B (plate freezer for 30 min, 4 ℃ storage), Group C (plate freezer for 120 min, - 30 ℃ storage), Group D (plate freezer for 120 min, - 4 ℃ storage), Group E (plate freezer for 30 min, - 4 ℃ storage), Group F (plate freezer for 120 min, 4 ℃ storage). At 0, 3, 5, 7 and 9 h after the completion of the preparation of cryoprecipitate, the changes and differences of the content of coagulation factor VIII (Fv III) and fibrinogen (FIB) in the six groups of samples were observed. The highest level of FvIII occurred in group B at 5 h with a concentration of (2.47±0.56) IU/mL, while the lowest level occurred in group A at 9 h with a concentration of (0.84±0.07) IU/mL. The highest level of FIB occurred in group A at 0 h with a concentration of (245.56±25.92) mg/200ml, while the lowest level occurred in group C at 9 h with a concentration of (195.16±13.98) mg/200ml. It is preferable that both FvIII and FIB levels are at a higher level at 9 h, with group B having more appropriate levels of FvIII and FIB. According to the analysis of variance, the content of FvIII will be affected by the storage temperature, time, quick-freezing method, and the interaction between quick-freezing method and time (P<0.05). The content of FIB will be affected by the effects of quick-freezing method, time, quick-freezing method and storage temperature (P<0.05). Different quick-freezing methods and storage temperatures affect FvIII and FIB in the cryoprecipitated fresh plasma. The optimal quick-freezing method is the plate quick-freezing machine for 30min, and the storage temperature is 4 ℃ . Under this condition, the content of coagulation factor in the cryoprecipitate decreased more slowly.