MorphologicaObservation of Specific Condensation Effect of Cholesterol on Dipalmitoyl Phosphatidyl Choline (DPPC) Monolayer by Dropping Method

生物物理化学(英文) Pub Date : 2016-09-19 DOI:10.4236/JBPC.2016.74009

T. Yokoyama, D. Yoshida, H. Mori, Masaya Okabe, Z. Shervani, K. Taga, Yasushi Yamamoto, Ayumi Sumino, Takehisa Dewa, M. Nango, Masato Yamamoto

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引用次数: 3

Abstract

Morphology of dipalmitoyl phosphatidyl choline (DPPC)-cholesterol (Chol) mixed monolayer formed on water surface by dropping method was investigated using surface tension measurement (STm), Brewster angle microscopy (BAM), and fluorescence microscopy (FM). STm showed strong condensation effect of Chol in fluidic DPPC monolayer. Excess area (Sex) from mean mixing state of DPPC and Chol was about twice larger than that by general compression method in the range from xC = 0.2 to 0.4 (xC: mole fraction of Chol). BAM and FM images showed clearly that the fluidic DPPC monolayer changed to condensed rigid monolayer due to the condensation effect of Chol. At more than xC = 0.3 DPPC-Chol mixed monolayer changed to condensed state similar to the Chol monolayer. These results support previous reports by compression method that Chol molecule demonstrates the strong condensation effect to the fluidic monolayer and also indicate that dropping method enables to form unique monolayer on the water surface.

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滴注法观察胆固醇对双棕榈酰磷脂酰胆碱(DPPC)单分子膜的缩聚效应

采用表面张力测量(STm)、Brewster角显微镜(BAM)和荧光显微镜(FM)研究了水滴法在水面形成的双棕榈酰磷脂酰胆碱(DPPC)-胆固醇(Chol)混合单层膜的形态。STm在流态DPPC单层中显示出较强的凝聚效应。在xC = 0.2 ~ 0.4 (xC: Chol的摩尔分数)范围内，DPPC与Chol平均混合状态下的剩余面积(性别)约为普通压缩法的2倍。BAM和FM图像清楚地显示，由于Chol的冷凝作用，流态DPPC单层转变为冷凝刚性单层。在大于xC = 0.3时，DPPC-Chol混合单层变为类似Chol单层的缩合态。这些结果支持了前人用压缩法报道的Chol分子对流体单层具有较强的冷凝作用，也表明滴入法能够在水面形成独特的单层。

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生物物理化学(英文)

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