The Effects of Chitosan-Hyaluronic Acid Blended Membrane on the Growth of Keratocytes

2009 2nd International Conference on Biomedical Engineering and Informatics Pub Date : 2009-10-30 DOI:10.1109/BMEI.2009.5305837

Zi-Ang Yao, Hai-Ge Wu, Ying Bai, Wei Qu, Yan Pan

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Abstract

In order to construct a suitable scaffold for corneal cells culture and transplantation in vitro, different chitosan- hyaluronic acid blended membranes were prepared and the compatibility of chitosan and hyaluronic acid in blended membranes was studied. Rabbit keratocytes were seeded onto the blended membranes surface and the effects of the blended membranes on cells attachment and metabolism were investigated. The results showed that chitosan and hyaluronic acid had good compatibility in blended membranes within a certain blended ratio. Within the blending ratio of 1:0.05, hyaluronic acid could reduce the damage of chitosan membrane to cells and improve the adhersion and proliferation of cells on blended membranes. So chitosan and hyaluronic acid had good compatibility within a certain blended ratio. Hyaluronic acid could improve the biocompatibility between cells and membranes. The blended membrane had potential to be used as a scaffold for corneal cells culture in vitro and a carrier for corneal cells transplantation. Keywords-chitosan; hyaluronic acid; blended membrane; keratocytes

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壳聚糖-透明质酸混合膜对角质细胞生长的影响

为了构建适合角膜细胞体外培养和移植的支架，制备了不同的壳聚糖-透明质酸混合膜，并研究了壳聚糖与透明质酸在混合膜中的相容性。将兔角质细胞植入混合膜表面，观察混合膜对细胞附着和代谢的影响。结果表明，壳聚糖与透明质酸在一定配比下，在共混膜中具有良好的相容性。在1:0.05的混合比例下，透明质酸可以降低壳聚糖膜对细胞的损伤，提高细胞在混合膜上的粘附和增殖能力。说明壳聚糖与透明质酸在一定配比下具有良好的相容性。透明质酸能改善细胞膜间的生物相容性。混合膜可作为角膜细胞体外培养的支架和角膜细胞移植的载体。Keywords-chitosan;透明质酸;混合膜;可见

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2009 2nd International Conference on Biomedical Engineering and Informatics

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