In vitro regeneration of an indigenous bamboo (Bambusa nutans) from internode and leaf explant

Abstract

Organogenic callus was successfully induced from pre-injured single shoots derived from in vitro multiplying cultures of Bambusa nutans. Plant growth regulator combinations of 2,4D (5 μ M), BAP (2.5 μ M) and ABA (1 μ M) proved to be more efficient in inducing callus, in 79.93% of cultures with an average of 346.13 mg of fresh weight callus, than auxins used alone or in combination with cytokinins. Induced callus was subsequently proliferated at a faster rate on multiplication medium (MS) supplemented with 2,4-D (5 μ M) and BAP (2.5 μ M). Organogenic callus was subsequently transferred to shoot regeneration medium. Efficient regeneration of shoot buds and their conversion into shoots was recorded on MS medium supplemented with BAP (5 μ M) and NAA (1.25 μ M) on which 18.11 buds were induced which proliferated into 10.31 shoots. Spontaneous regeneration of roots on shoots was evidenced on regeneration medium itself.