Electrophoretic Profile of Protein and Isozymes in Leaf Gall Of Pongamia Pinnata (L.)

Abstract

In the present investigation the electrophoretic studies of protein and isozymes (peroxidase and polyphenol oxidase) in leaf galls of Pongamia pinnata induced by Aceria pongamiae were carried out. The results showed some variation in SDS-protein and isozymes activities banding patterns among leaf galls and normal leaf. Total twenty bands were observed in each lane of galled leaf sample, in comparison one less band was observed in normal leaf crude protein profiling of experimental plant. We observed that the intensity of protein bands was higher in galled leaf as compare to normal leaf protein. Intensity of polyphenol oxidase and peroxidase activity was found to be very high in galled leaves as compared to their normal counterparts.