FTIR and GCMS analysis of Euphorbia hirtaL. and its In-vitroAntibacterial and Antioxidant Activities

Indo Global Journal of Pharmaceutical Sciences Pub Date : 2022-01-01 DOI:10.35652/igjps.2022.12009

D. Kain, Suresh Kumar, Vandana, Amrita Suryavanshi, Atul Arya

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Abstract

Introduction: Euphorbia hirtaL. is a common weed plant belongs to family Euphorbiaceae. Thepresent study brings out the importance of weed plants like E. hirtain medicine and health thecare system. Material and Methods:Plant E. hirtahas been collected from thegarden of Department of Botany, University of Delhi and plant leaves were extractedin ethanol by thesoxhlet method. Antibacterial screening has been done by disc-diffusion method. Theantioxidant potential has been determined by TPC, TFC, FRAP, Total antioxidants through phosphomolybdate assay and by DPPH radical scavenging activity. FTIR and GCMS analysis havebeen done for characterization.Result and Discussions:Plant E. hirtashowed significant antibacterial activity against Escherichia coli,Staphylococcus aureus, Bacilus subtilisand Pseudomonas aeruginosawith maximum ZOI diameter 19±0.5mmagainst S.aureusandP.aeruginosaboth. Plant E. hirtaL. showedsignificant antioxidant potential with 196.32 mg/g, 39.7133 mg/g, 595.99 mg/g, 525.84 mg/g values for TPC, TFC, FRAP activity and total antioxidants through phosphomolybdate assay respectively. Plant E. hirtaalso showedsignificant radical scavenging activity against DPPH free radical with amaximum % inhibition 68.505% at 1000μg/ml concentration. FTIR and GCMS of plant E. hirtahave confirmed the presence of various active functional groups and compounds.Conclusion:The current study justifies the importance of E. hirta L. a common weed asasource of natural antioxidants having antibacterial potential and its role in herbal medicines.©2022iGlobal Research and PublishingFoundation. All rights reserved.

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大戟的FTIR和GCMS分析。及其体外抗菌和抗氧化活性

简介:大戟属植物。是一种常见的杂草植物，属于大戟科。本文的研究结果揭示了黄芪等杂草植物在医药和保健系统中的重要作用。材料与方法:采自印度德里大学植物学系植物园内，采用乙醇提取法提取植物叶片。采用圆盘扩散法进行抗菌筛选。通过磷酸钼酸盐测定TPC、TFC、FRAP、总抗氧化剂和DPPH自由基清除活性测定其抗氧化能力。FTIR和GCMS分析已完成表征。结果与讨论:对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌和铜绿假单胞菌均有显著的抑菌活性，对金黄色葡萄球菌和铜绿假单胞菌的ZOI最大直径为19±0.5mm。植物E. hirtaL。经磷钼酸盐测定，其TPC、TFC、FRAP活性和总抗氧化剂分别为196.32 mg/g、39.7133 mg/g、595.99 mg/g、525.84 mg/g，具有显著的抗氧化潜力。在浓度为1000μg/ml时，对DPPH自由基的抑制率最高达68.505%。经红外光谱和气相色谱分析，证实其含有多种活性官能团和化合物。结论:本研究证实了一种常见的杂草——赤藓属植物作为一种具有抗菌潜力的天然抗氧化剂的重要性及其在中草药中的作用。©2022全球研究与出版基金会。版权所有。

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Indo Global Journal of Pharmaceutical Sciences

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