Investigations on microbial fermentation of hemicellulose hydrolysate for xylitol production.

A. Bhattacharya, A. Sadhukhan, A. Ganguly, P. K. Chatterjee
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引用次数: 11

Abstract

A crude trial on microbial fermentation of dilute acid treated hemicellulose hydrolysate of water hyacinth (Eichhornia crassipes) biomass was performed in batch culture in a BOD rotary shaker using four different species as Candida shehatae, Pichia stipitis, Candida tropicalis and Debaromyces hansenii under various parameters to check the optimal xylitol production. The hydrolysate was concentrated using a vacuum evaporator at 70C and detoxified using activated charcoal (5 %) which efficiently removed 83 % of inhibitory phenolic biproducts. Effect of nutrients concentration, pH (4.5, 5.5, 6.5), temperature (20, 30, 40C) and agitation speed (150, 200, 250 rpm) on growth of different yeast and xylitol production was studied and compared. The optimal xylitol yield was found to be 0.65 g xylitol/g of xylose from Candida tropicalis at 30C, pH 5.5 with an agitation of 200 rpm, 2.5 g/L ammonium sulphate as a nutrient and 0.615 xylitol/g of xylose from Pichia stipitis respectively after 72 h of incubation. Keyword: Microbial fermentation, water hyacinth biomass, xylitol production, yeast, detoxification.
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半纤维素水解物微生物发酵生产木糖醇的研究。
以水葫芦(Eichhornia crassipes)生物质经稀酸处理的半纤维素水解产物为原料,在BOD旋转摇床上进行了批量发酵试验,选取了4种不同的菌种,分别是shehat念珠菌、Pichia stipitis、Candida tropicalis和Debaromyces hansenii,在不同的参数下考察了木糖醇的最佳产量。水解产物用真空蒸发器在70℃下浓缩,用活性炭(5%)解毒,有效去除83%的抑制性酚类双产物。研究并比较了营养物质浓度、pH(4.5、5.5、6.5)、温度(20、30、40℃)和搅拌速度(150、200、250 rpm)对不同酵母生长和木糖醇产量的影响。最佳木糖醇产率为:在30℃、pH 5.5、搅拌转速200 rpm、硫酸铵2.5 g/L、毕赤酵母木糖0.615 g/ g条件下,热带念珠菌木糖产率为0.65 g/ g。关键词:微生物发酵,水葫芦生物量,木糖醇生产,酵母,解毒
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