Study on Phytochemical Profile and Antioxidant Activity of Achyranthes aspera Whole Plant

International Journal of Biochemistry Research and Review Pub Date : 2021-05-28 DOI:10.9734/IJBCRR/2021/V30I230251

N. Manandhar, Kuber Bajgain, A. Neupane

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引用次数: 5

Abstract

This study aims to establish qualitative as well as quantitative phytochemical profiles and determine the free radical scavenging activity of phytochemical constituents of the entire plant of Achyranthes aspera Linn. The extraction of dried plant material was carried out by cold maceration with methanol followed by partitioning with ethyl acetate. Preliminary phytochemical screening of the plant was carried out by performing various chemical tests. Phenolic and flavonoid contents were determined by Folin-Ciocalteu and Aluminum chloride methods, respectively. Antioxidant activity was studied using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. The result of study revealed the presence of flavonoids, alkaloids, terpenoids, phytosterols, tannins, saponins, phenolic compounds, and carbohydrates. Total phenolic content, total flavonoids content, and antioxidant activity of the extract were found as 209.007 μg GA/mg, 17.59 μg QE/mg, and 25.12% (100 μg/mL), respectively. The study showed that plant could be a source of antioxidant compounds.

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牛膝全株植物化学特征及抗氧化活性研究

本研究旨在建立牛膝草(Achyranthes aspera Linn)全株植物化学成分的定性和定量图谱，并测定其清除自由基的活性。用甲醇冷浸渍法提取干燥的植物原料，然后用乙酸乙酯进行萃取。通过各种化学试验对该植物进行了初步的植物化学筛选。采用福林- ciocalteu法和氯化铝法分别测定酚类和类黄酮含量。采用2,2-二苯基-1-苦味酰肼(DPPH)自由基清除法研究了其抗氧化活性。研究结果显示黄酮类、生物碱、萜类、植物甾醇、单宁、皂苷、酚类化合物和碳水化合物的存在。提取物总酚含量为209.007 μg GA/mg，总黄酮含量为17.59 μg QE/mg，抗氧化活性为25.12% (100 μg/mL)。研究表明，植物可能是抗氧化化合物的来源。

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International Journal of Biochemistry Research and Review

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