Antifungal activity screening and HPLC analysis of crude extract from Tectona grandis, Shilajit, Valeriana wallachi.

Abstract

The antifungal activity of methanolic crude extract of Tectona grandis, Shilajit, Valeriana wallachi was investigated against Alternaria cajani, Curvularia lunata, Fusarium sp., Bipolaris sp. and Helminthosporium sp. at different concentrations (1000, 2000, 3000, 4000 and 5000 �og/ml). Better antifungal activity was observed with the extracts of Valeriana wallachi, that showed excellent inhibitory activity against Helminthosporium sp. (96.15%) followed by Shilajit extract against Alternaria cajani (95.12 %) and Helminthosporium sp. (95.00 %) at concentration of 5000 �og/ml. Among different fungi tested Bipolaris sp. and Fusarium were found to be more sensitive to crude extract when compared to others. The increase in the production of phenolics in the extract can be correlated with the induction of resistance in treated plants against phytopathogenic fungi. HPLC analysis of the crude extract of medicinal plants showed four different Phenolic acids (Tannic acid, Gallic acid, Ferulic acid and Caffeic acid). The results of the study provide scientific basis for the use of the plant extract in the future development as antioxidant, antibacterial, antifungal and anti-inflammatory agent.